Mutation is a very common biological phenomenon, but the detection of mutation is a complex and tedious task, especially for higher plants and animals with large genomes.

Operation method

mutation detection

Materials and Instruments

Drosophila melanogaster strain : Wild type ClB strain
Drosophila culture medium Ether
X-ray instrument, autopsy mirror, constant temperature incubator, bio-gel capsule, culture bottle and anesthesia bottle.

Move

1. Male wild-type Drosophila were placed in biogelatin capsules and treated with different doses of X-rays.

2. The treated male Drosophila were crossed with female virgin flies of ClB strain, 5 pairs were inserted into each bottle, and the males were separated by different doses of X-ray treatment.

3. The parents could be released after 1d.

4.Self-cross the F1 Drosophila rod-eye females with normal males in pairs, and place each pair in a small culture bottle.

5. Release the single pair of parents after 5-7d.

6. Anesthetize the F2 adults gradually, batch by batch, from the time they fledge, and observe the F2 male Drosophila.

Observe the sex and traits of F2 fruit flies carefully, if there are normal males in F2, it means that the radiation treatment did not cause gene mutation; if there are no males, it means that a lethal mutation has occurred on the X chromosome of the male flies to be tested; if there are mutant males, the mutation is induced by the radiation treatment.

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