The fructose of seminal plasma is co-cooled with resorcinol in an acidic environment at 90℃ to produce a red compound, the color of which is directly proportional to the fructose content. This experiment is derived from the experiment guide of Mudanjiang Medical College undergraduate 5-year testing program.

Operation method

Determination of fructose in seminal plasma

Principle

Fructose in concentrate is co-cooled with resorcinol in an acidic environment at 90°C to produce a red compound, the color of which is proportional to the fructose content.

Materials and Instruments

Aldehyde resin Ba(OH)2 Resorcinol solution Hydrochloric acid

Move

I. Experimental reagents: ① aldehyde resin 0.175mmol / L _ZnSO4

1. 0.6 mol / L Ba (OH) ₂

2. lg / L resorcinol solution

3. 10mol / L HCI

fructose standard solution: storage solution (25m mol / L) finely weighed unification of 450.0mg of fructose in 100ml volumetric flasks, add 2g / L benzoic acid solution to the scale of the solution; application of the solution (0.25mmo / lL) storage solution lml plus 2g / L benzoic acid solution to 100ml mixed. 0.25mmo/lL) storage solution lml plus 2g / L benzoic acid solution to 100ml mix.

Second, the experimental operation:

1. Will be liquefied semen centrifuged at 3500r/min for 10 minutes.

2. Take the supernatant seminal plasma 0.1ml plus distilled water 2.9ml, and then add 0.6 mol / L Ba (OH) 2 0.5mll mixing, and then add 0.175mol / L _ZnSO4. 0.5ml mixing fully, let it stand for 5 minutes centrifugation, and take the supernatant standby.2. take the small test tube 3 according to the following table plus be;



3. Add liquid mixing and set 90 ℃ bath for 10 minutes to take the effluent cooling, with 490nm wavelength colorimetric

Third, the experimental calculations: fructose (mg / L) = (determination of the tube absorbance / absorbance of the standard tube) x 2000

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