What Is Diosmetin?

Diosmetin (CAS 20126-59-4) is a naturally occurring O-methylated flavone with the molecular formula C₁₆H₁₂O₆ and a molecular weight of approximately 300.26 g/mol. It is classified as a flavonoid aglycone—specifically, it is the aglycone form of diosmin, a well-known bioflavonoid. Structurally, diosmetin features a flavone backbone bearing hydroxyl groups at positions 5 and 7 and a methoxyl group at the 4′-position of the B-ring.

Diosmetin is distributed widely in the plant kingdom, occurring in citrus fruits, members of the genus Mentha, and numerous other botanical sources. Its relatively simple structure and distinct biological profile make it a popular research tool for studying flavonoid pharmacology.

Solubility and Handling of Diosmetin

Diosmetin is practically insoluble in water due to its hydrophobic flavone core. It is soluble in organic solvents such as dimethyl sulfoxide (DMSO) and ethanol, making DMSO the most common vehicle for preparing stock solutions in cell-based assays. When preparing solutions, gentle warming and vortexing may aid dissolution. It is recommended to prepare fresh working solutions by diluting DMSO stocks into aqueous buffer immediately before use, ensuring that the final DMSO concentration in assay wells remains below cytotoxic thresholds (typically ≤0.1% v/v for sensitive cell lines).

Researchers should handle Diosmetin following standard laboratory safety practices: wear appropriate personal protective equipment (gloves, lab coat, eye protection), and consult the Safety Data Sheet (SDS) before use. Avoid prolonged exposure to strong light, which may degrade the compound.

Biological Activities and Research Applications

Diosmetin has been investigated across multiple areas of biomedical research. The principal biological activities reported in the scientific literature include:

• Antioxidant activity: Diosmetin can scavenge reactive oxygen species (ROS) through its phenolic hydroxyl groups, and has been studied in models of oxidative stress.
• Anti-inflammatory activity: Research suggests diosmetin may modulate inflammatory signaling pathways, including inhibition of pro-inflammatory cytokine production.
• Anticancer/antiproliferative activity: In vitro studies have examined diosmetin's effects on the proliferation, apoptosis, and cell cycle of various cancer cell lines.
• Enzyme inhibition: Diosmetin has been reported to inhibit certain kinases and other enzymes relevant to disease pathways.
• Cardioprotective and hepatoprotective effects: Preclinical studies have explored protective effects in cardiac and hepatic injury models.

These activities make Diosmetin a useful tool compound in drug discovery screening, mechanistic pathway studies, and natural product research.

Storage and Stability

To maintain compound integrity, Diosmetin should be stored as a solid in a cool, dry location, protected from light and moisture. Storage at −20 °C under desiccated, inert conditions is commonly recommended for long-term preservation, though researchers should always refer to the product-specific Certificate of Analysis (CoA) and SDS for the exact recommended storage conditions supplied by Aladdin Scientific.

Once dissolved, stock solutions in DMSO should be aliquoted to avoid repeated freeze-thaw cycles, which can compromise compound stability. Unused aliquots should be stored at −20 °C and shielded from light. Working solutions in aqueous media should be prepared fresh and used promptly.

Ordering Diosmetin from Aladdin Scientific

Aladdin Scientific supplies Diosmetin under SKU D137256. Researchers and procurement staff can place orders directly through the Aladdin Scientific website. When ordering, please verify the purity grade and quantity against your experimental requirements, and request a Certificate of Analysis (CoA) to confirm lot-specific purity and identity data.

For bulk quantities, custom purity specifications, or technical questions about Diosmetin, contact Aladdin Scientific customer support. Standard shipping conditions and return/refund policies are detailed in the Help and Customer Service section of the Aladdin website.