Recipe

1.Dissolve 5.3g of Na2CO3 in 900ml distilled H2O

2.Dissolve 4.2g of NaHCO3 in the solution from step 1

3.Dissolve 1g sodium azide in the solution from step 2

4.Dissolve 5g BSA in solution from step 3

5.Alternatively you can also use 50g non-fat dry milk for blocking solution

6.pH to 9.6

7.Adjust volume to 1L with additional distilled H2O