This experiment is from the official website of Parasitology, School of Medicine, Shandong University.

Operation method

Morphological observation experiments of Pneumocystis carinii sp.

Principle

The large trophozoites of Pneumocystis carinii were variable in shape, 2-8 μm long, with only one dark purple nucleus and light blue cytoplasm after staining with Kirschner's solution. The small trophozoites were 1~1.5μm in size, spherical or amoeboid. Pre-encapsulation, 3~5μm long, egg-shaped, with several filamentous pseudopods, a bunch of mitochondria and 1~8 nuclei were visible in the encapsulation with phase contrast microscopy with 8 ascospores, and the nuclei were visible after special staining.

Move

Encystment: Biopsy of encystment taken from respiratory and lung tissues, spherical, 4-6 μm, with 8 ascospores under phase contrast microscope.

Pneumocystis carinii encapsulation

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