It is used to observe whether the bacteria can ferment glucose, lactose and decompose protein to produce hydrogen sulfide, which is convenient for the preliminary identification of bacteria. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for testing majors.

Operation method

Experiments on the preparation of iron agar medium with Kirschner's disaccharide

Principle

It is used to observe whether the bacteria can ferment glucose, lactose and decompose proteins to produce hydrogen sulfide, which facilitates the preliminary identification of bacteria.

Move

Ingredients:

Peptone or polyvalent peptone: 20g

Beef infusion: 3.0 g

Yeast powder: 3.0 g

Lactose: 10.0 g

NaCl: 5 g

Glucose: 4 g

Ferric ammonium citrate: 0.5 g

Sodium thiosulfate: 0.5 g

Agar: 12-15 g

Phenol red: 0.025 g

Distilled water: 1000 ml

PH 7.4

Preparation:

Mix the above components, add temperature to dissolve, adjust the PH divided into test tubes. 121 ℃ 15min sterilization, put the slant cooling standby.

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