The experimental method was obtained from the official website of the Fourth Military Medical University

Operation method

Experiments on venous blood sampling from the anterior and posterior limbs of dogs and cats

Materials and Instruments

Dogs Cats
Small animal lab bench Syringes Alcohol

Move

The medial subcutaneous cephalic vein was chosen for the forelimb, located in the anterior part of the forelimb and traveling medially in the lower 1/3; the lateral saphenous vein was chosen for the hindlimb, located in the lateral part of the tarsus and subcutaneously 5-10 cm above the tarsus, traveling obliquely from anterior to posteriorly superior. When collecting blood, the dog was first immobilized, and a tourniquet was tied above the puncture site to fill the vein. The operator holds a syringe to collect blood.

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