Mouse urine is more difficult to collect because of its high concentration and low volume, and the main composition and concentration are related to the degree of shock of the mouse at the time of collection, so it is difficult to be used consistently for biochemical assays, and has a lower degree of general applicability. Feces can be used mainly for the identification of intestinal flora culture to determine the intestinal microbial composition and nutritional status of mice.

Operation method

Feces and urine collection experiment

Materials and Instruments

Equipment: mice.

Move

The basic process of fecal and urine collection experiments can be divided into the following steps:
(a) Urine collection in mice under non-invasive conditions is generally done in the following ways.
1. Complete free urination
The mice are placed in an enclosed space lined with plastic film under careful observation, and when they urinate the urine is sucked up with a clean lance tip and accumulated. Multiple mice can be separated by cardboard on a full sheet of plastic wrap at the same time, and urine can be collected at the same time until the desired amount is reached.
2. Pressing on the abdomen to urinate
Usually two people need to work together to accomplish this. One person gently grasps the mouse and presses moderately on the abdomen where the bladder is located. The mouse will usually urinate voluntarily while the other person collects the urine with a clean EP tube. This method is more commonly used because of its simplicity, but the disadvantage is that the amount of urine collected cannot be controlled at will.
3. Reflex Urination
The mouse is picked up by the tail and placed in an inverted position, and in this body position the mouse will urinate on its own, with a suitable tube to catch it.
In addition to the above methods, there are invasive methods such as cystocentesis, which are not commonly used.
(ii) Fecal collection
Fecal collection in mice is generally done using metabolic cages, where feces are collected naturally under normal physiological conditions, and can be used in conjunction with food intake to analyze the excretory function of the mouse food. Freshly separated feces under compression can be used for common bacterial cultures, mostly used by veterinarians to analyze diseases such as intestinal dysfunction in mice.

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