Chemical Identity and Structure

H-Gly-Gly-Gly-OEt.HCl, also known as triglycine ethyl ester hydrochloride, is a synthetic tripeptide composed of three glycine residues in which the C-terminal carboxyl group is protected as an ethyl ester and the N-terminus is free (unprotected). The compound is supplied as the hydrochloride salt to enhance stability and handling convenience.

Glycine (Gly) is the simplest amino acid, lacking a chiral center, making triglycine derivatives achiral and particularly useful as model substrates in mechanistic and structural studies. The ethyl ester moiety (OEt) renders the C-terminus stable under neutral conditions while remaining amenable to hydrolysis under acidic or basic conditions, a feature exploited in peptide elongation strategies.

The molecular formula is C₁₀H₂₁ClN₃O₄ (free base: C₁₀H₁₉N₃O₄). Researchers should confirm the exact molecular weight and purity specification from the current product certificate of analysis (CoA) provided with SKU H734012.

Safe Handling and Storage

As with all laboratory reagents, appropriate personal protective equipment (PPE) should be worn when handling H-Gly-Gly-Gly-OEt.HCl. This includes laboratory coat, nitrile gloves, and safety goggles. The hydrochloride salt form means the material is acidic in solution; avoid inhalation of dust and prevent contact with skin and mucous membranes.

Storage recommendations: keep the reagent in a tightly sealed container in a cool, dry, well-ventilated area away from direct sunlight and moisture. Hygroscopic behavior is possible for hydrochloride salts of peptides; desiccants may be used to maintain product integrity. Always consult the Safety Data Sheet (SDS) provided by Aladdin Scientific for comprehensive hazard and first-aid information specific to this product.

Disposal should follow local regulations for chemical waste. Do not dispose of down drains without treatment where prohibited by applicable regulations.

Preparation of Working Solutions

H-Gly-Gly-Gly-OEt.HCl is generally soluble in water and common polar organic solvents including methanol, ethanol, and DMSO. For aqueous stock solutions, dissolve the compound in high-purity water (HPLC-grade or Milli-Q) at the desired concentration. For sensitive assays, filter the solution through a 0.22 µm membrane filter prior to use.

When preparing solutions in organic solvents, ensure the solvent is compatible with downstream assay reagents and biological systems. Stock solutions should be prepared fresh or aliquoted and stored at −20 °C to minimize hydrolysis of the ethyl ester over time. Repeated freeze-thaw cycles should be minimized to preserve reagent integrity.

Always verify solution pH when working with the hydrochloride salt form, particularly in cell-based or enzyme-based assays where pH stability is critical. Buffering with an appropriate biological buffer (e.g., PBS, HEPES) is recommended.

Laboratory Applications

H-Gly-Gly-Gly-OEt.HCl serves as a versatile reagent in several areas of chemical biology and synthetic chemistry:

• Peptide Synthesis: As a C-terminally protected tripeptide, it acts as a convenient building block for the synthesis of longer peptide chains via solution-phase or semi-automated methods. The ethyl ester can be selectively deprotected to reveal a free carboxylate for further coupling.
• Enzyme Substrate Studies: Triglycine-based substrates and their derivatives are classical substrates for protease and peptidase activity assays. Researchers use such reagents to characterize enzyme specificity and kinetics.
• Model Peptide Research: The absence of side chains in glycine makes triglycine an ideal minimal model for studying backbone conformational dynamics, hydrogen bonding patterns, and hydration of peptide bonds.
• Calibration and Method Development: Defined tripeptide esters are sometimes used as reference standards in liquid chromatography (HPLC, LC-MS) method development and instrument calibration.

Quality, Ordering & Regulatory Notes

Aladdin Scientific's H-Gly-Gly-Gly-OEt.HCl (SKU: H734012) is offered as a research-grade reagent. Each lot is accompanied by a Certificate of Analysis (CoA) detailing identity confirmation, purity, and physical characteristics. Researchers and procurement teams should request the CoA for each lot received to confirm compliance with experimental requirements.

This product is intended solely for research and development purposes and is not approved for clinical, diagnostic, or therapeutic use in humans or animals. Regulatory requirements for use, import, and storage may vary by country and institution; consult your institutional biosafety or regulatory officer as appropriate.

For pricing, availability, bulk quantities, or custom specifications, contact Aladdin Scientific customer service or visit the product page for SKU H734012 on the Aladdin Scientific website.