Target Biology: What Is HBEGF?

Heparin-Binding EGF-Like Growth Factor (HB-EGF) is a member of the epidermal growth factor (EGF) family of proteins. It is synthesized as a transmembrane precursor (proHB-EGF) that can be proteolytically cleaved to release a soluble, biologically active form. HB-EGF signals primarily through the ErbB1 (EGFR) and ErbB4 receptors, activating downstream pathways including RAS/MAPK and PI3K/AKT, which govern cell proliferation, survival, migration, and differentiation.

HB-EGF plays important roles in wound healing, cardiac hypertrophy, tumor progression, and embryonic development. Its dual membrane-bound and soluble forms allow it to act in both juxtacrine and paracrine signaling modes, making it a versatile regulator of tissue homeostasis and disease.

Antibody Overview: HBEGF Mouse mAb (Ab349002)

The HBEGF Mouse mAb (catalog number Ab349002) is a mouse monoclonal antibody raised against HB-EGF. This antibody is referenced under clone KC-2503 (absea) and is offered by Aladdin Scientific as a reliable reagent for detecting HBEGF in research settings.

As a monoclonal antibody, it provides high specificity and lot-to-lot consistency, which are critical for reproducible results across experiments. Researchers studying EGF receptor signaling, tumor microenvironment, cardiac biology, or developmental processes will find this reagent valuable for reliably identifying HB-EGF protein expression and distribution.

Recommended Applications

Monoclonal antibodies targeting HBEGF are commonly employed in a range of immunological and biochemical assays. While researchers should consult the official datasheet for fully validated application details, typical applications for anti-HBEGF monoclonal antibodies include:

• Western Blotting (WB): Detection of HBEGF protein in cell or tissue lysates under denaturing conditions.
• Immunofluorescence (IF): Visualization of HBEGF localization in fixed cells or tissue sections.
• Immunohistochemistry (IHC): Assessment of HBEGF expression in formalin-fixed, paraffin-embedded (FFPE) tissue samples.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative measurement of HBEGF levels in biological fluids or culture supernatants.
• Immunoprecipitation (IP): Pull-down of HBEGF protein complexes for downstream analysis.

Always refer to the product datasheet for confirmed species reactivity, recommended dilutions, and appropriate controls before proceeding with experiments.

Storage, Handling & Quality Considerations

Proper storage and handling of monoclonal antibodies are essential to maintain activity and specificity. General best practices for antibody reagents include:

• Store at –20 °C for long-term storage; avoid repeated freeze–thaw cycles by aliquoting upon first use.
• Keep working stocks at 4 °C for short-term use (typically up to one month), protected from light.
• Centrifuge briefly before opening to ensure complete recovery of the antibody solution.
• Use appropriate positive and negative controls in every experiment to validate assay performance.

For specific storage conditions, formulation details, concentration, and expiry guidance, consult the official product datasheet for Ab349002 available from Aladdin Scientific. Contact technical support for application-specific optimization assistance.