When the plant tissues were in contact with the highly concentrated sugar solution, the bound water remained in the tissues because it was adsorbed by the protoplasmic colloidal particles; the free water was extravasated into the sugar solution along the water potential gradient because it was not adsorbed by the protoplasmic colloidal particles, so that the concentration of the sugar solution was reduced. After the tissues were immersed in the sugar solution for a certain time, the free water content in the tissues could be calculated based on the decrease in the concentration of the sugar solution, while the bound water content could be calculated by drying the plant tissues to find the total water content and then subtracting the free water content.

Operation method

Experiments for the determination of the total water content of plant tissues

Move

I. Drying and weighing method

1. Take 3 weighing flasks, numbered in sequence, and weigh the empty flasks separately.

2. Take the same plant material as above and drill the discs with a hole punch. Immediately put it into the weighing bottle (50 slices for each bottle randomly), cover it with a lid, and accurately weigh the fresh weight of the sample to be measured (if the plant to be measured is a tuber, cut it into round slices of about 1㎜ thickness, and accurately weigh 1 g of the sample to be measured).

3. put 3 bottles of weighed samples into the oven 100-105 ℃ drying, and then placed in the desiccator cooling constant weight weighed dry weight. The total water content of the plant tissue (﹪) was calculated according to the formula (1).

Second, rapid moisture analyzer method

1. Take the same plant leaves, with a perforator drilling discs, immediately weighed samples to be measured 1 ~ 5g (if the plant under test is a tuber, first with a perforator drilling round strip, take about 2 cm long (1 ~ 5g ), and then cut with a razor blade into a disc with a thickness of about 1mm). Total plant tissue moisture content (%) was determined directly with a rapid moisture analyzer.

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