ICAM2 Mouse mAb: FAQs for Research Use
ICAM2 Mouse mAb: FAQs for Research Use
ICAM2 Mouse mAb is a monoclonal antibody raised in mouse targeting intercellular adhesion molecule 2 (ICAM2), designed to support a range of immunological research applications including detection and functional studies of this key cell-surface adhesion protein.
- Product Name
- ICAM2 Mouse mAb
- SKU
- Ab349023
- Host Species
- Mouse
- Target Antigen
- ICAM2 (Intercellular Adhesion Molecule 2)
- Antibody Type
- Monoclonal Antibody (mAb)
- Supplier Reference
- absea#KC-26799
What Is ICAM2 and Why Is It Studied?
ICAM2 (Intercellular Adhesion Molecule 2) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is constitutively expressed on endothelial cells, lymphocytes, monocytes, and platelets, where it mediates cell–cell and cell–extracellular matrix adhesion. ICAM2 serves as a ligand for the integrin LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), playing important roles in leukocyte transendothelial migration, immune surveillance, and angiogenesis. Research into ICAM2 spans immunology, vascular biology, inflammation, and cancer biology, making high-quality antibody reagents essential tools for these investigations.
Key Specifications of ICAM2 Mouse mAb (Ab349023)
The ICAM2 Mouse mAb (SKU: Ab349023) is a mouse-derived monoclonal antibody targeting human ICAM2. Below is a summary of key specifications. Note that full datasheet details—including exact clone designation, isotype, immunogen sequence, recommended dilutions, and validated application list—should be confirmed on the official Aladdin Scientific product page or by contacting technical support, as the supplier reference (absea#KC-26799) may carry additional lot-specific information.
- Host: Mouse
- Target: ICAM2 (Intercellular Adhesion Molecule 2)
- Antibody Format: Monoclonal
- Supplier Reference: absea#KC-26799
- SKU: Ab349023
Researchers are advised to download the current datasheet for the most up-to-date application notes and lot-specific quality control data.
Common Applications for ICAM2 Mouse mAb
Mouse monoclonal antibodies against ICAM2 are generally employed across a range of standard immunological assays. Typical applications for antibodies of this class include:
- Flow Cytometry (FC): Detection of ICAM2 surface expression on endothelial cells, lymphocytes, and platelets.
- Immunofluorescence (IF): Localization of ICAM2 in fixed cell or tissue sections.
- Western Blot (WB): Detection of ICAM2 protein in cell lysates under denaturing conditions (suitability depends on epitope and clone).
- Immunohistochemistry (IHC): Assessment of ICAM2 expression in formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections.
- Immunoprecipitation (IP): Enrichment of ICAM2 protein complexes from cell lysates.
Always verify which applications have been experimentally validated for Ab349023 by consulting the product datasheet or contacting Aladdin Scientific technical support before use.
Storage, Handling, and Stability
Proper storage and handling are critical to maintaining antibody activity. While specific storage conditions for Ab349023 should be confirmed on the product datasheet, general best practices for mouse monoclonal antibodies include:
- Short-term storage (up to 1 month): Store at 4 °C to minimize freeze–thaw cycles.
- Long-term storage: Aliquot and store at −20 °C or −80 °C. Avoid repeated freeze–thaw cycles, as these can reduce antibody activity.
- Carrier protein: Many formulations include BSA and/or glycerol as stabilizers; check the datasheet for exact formulation details.
- Avoid: Prolonged exposure to light, heat, or repeated temperature fluctuations.
If the antibody arrives lyophilized, reconstitute according to the datasheet instructions using sterile distilled water or the recommended buffer, and allow to equilibrate before use.
Ordering and Technical Support
ICAM2 Mouse mAb is available from Aladdin Scientific under SKU Ab349023. To place an order or request a quotation, visit the Aladdin Scientific website and search by SKU or product name. For bulk or custom orders, contact the Aladdin Scientific sales team directly.
For technical questions regarding experimental design, optimization, or troubleshooting, Aladdin Scientific's technical support team can be reached via the website contact form or email. When contacting support, please have the following information ready:
- Product SKU (Ab349023) and lot number
- Application and species/sample type being tested
- Protocol details including dilution, detection method, and blocking conditions
- Description of the issue or question
Aladdin Scientific also accepts returns and offers refunds in accordance with its return policy; please refer to the Returns & Refunds section of the website for full terms.
Frequently asked questions
What is ICAM2 Mouse mAb (Ab349023) and what does it target?
ICAM2 Mouse mAb (Ab349023) is a mouse monoclonal antibody designed to specifically bind intercellular adhesion molecule 2 (ICAM2), a transmembrane glycoprotein of the immunoglobulin superfamily expressed on endothelial cells, lymphocytes, monocytes, and platelets. It is intended for use in immunological and cell biology research applications.
Which experimental applications has Ab349023 been validated for?
The validated applications for Ab349023 should be confirmed by consulting the official Aladdin Scientific product datasheet, as experimental validation details (e.g., flow cytometry, immunofluorescence, western blot, IHC, or IP) are lot- and clone-specific. Please download the current datasheet from the Aladdin Scientific website or contact technical support for confirmation.
How should I store ICAM2 Mouse mAb to maintain its activity?
For short-term use (up to one month), store at 4 °C. For long-term storage, aliquot and keep at −20 °C or −80 °C to avoid repeated freeze–thaw cycles that can reduce antibody performance. Always refer to the specific storage instructions on the Ab349023 product datasheet for formulation-specific guidance.
What species reactivity does ICAM2 Mouse mAb (Ab349023) have?
Species reactivity information for Ab349023 should be verified on the official product datasheet available from Aladdin Scientific. Monoclonal antibodies have defined reactivity profiles determined by their immunogen; please contact Aladdin Scientific technical support if you need confirmation for a specific species or sample type.
How do I order Ab349023 and what is the return policy?
Ab349023 can be ordered directly from the Aladdin Scientific website by searching the SKU. For bulk pricing or quotations, contact the sales team. Returns and refunds are handled according to Aladdin Scientific's published return policy; please refer to the Returns & Refunds section of the website for eligibility criteria and procedures.
References
- Springer TA. Adhesion receptors of the immune system. Nature. 1990;346(6283):425-434. doi:10.1038/346425a0
- Aladdin Scientific. ICAM2 Mouse mAb product page (Ab349023). Accessed 2024.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
危险品化学品经营许可证（带存储）