In the presence of mouse IL-4 (Mil-4), IL-10 stimulated proliferation of the D36 mouse mast cell line. In contrast, single mIL4 or hIL-10 had only very low proliferation-stimulating activity.

Operation method

IL-10 biological activity assay

Materials and Instruments

Mouse IL-4
FCS-IMDM culture solution rh IL-10 standard 3H-TdR
Centrifuge Incubator

Move

D36 cell suspension preparation①↓Inoculate cells in a 96-well culture plate②.↓
Add the sample to be tested and the standard ③↓plus 3H-TdR④↓
Cells were collected and the amount of 3H-TdR adulteration was determined ⑤↓Plot the standard curve and calculate the activity units of IL-10 of the samples to be tested ⑥

Caveat

①Take the vigorously growing D36 cells and wash them twice with IMDM culture medium, suspend them in 20% FCS-IMDM culture medium, and make a concentration of 2×104/ml.②~⑥ Refer to IL-4 assay.

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