What Is IL2RB (CD122) and Why Does It Matter?

IL2RB, commonly designated CD122, encodes the beta subunit of the interleukin-2 receptor (IL-2R). Unlike the alpha chain (CD25), which confers high-affinity IL-2 binding only on activated T cells, the beta chain is constitutively expressed on resting T cells, NK cells, and certain B cell subsets, forming a key component of the intermediate-affinity IL-2 receptor complex (IL-2Rβγ).

Critically, the IL2RB subunit is also shared by the IL-15 receptor complex, making it a nodal point for at least two major cytokine signaling axes that govern lymphocyte survival, proliferation, and effector function. Signal transduction through IL2RB relies on its association with JAK1 and downstream activation of the STAT5 pathway, which drives gene programs essential for immune homeostasis and anti-tumor immunity.

Dysregulation of IL2RB expression has been documented in various hematological malignancies and autoimmune conditions, underscoring its relevance as both a biomarker and a therapeutic target in translational immunology research.

IL2RB Mouse mAb: Product Overview and Key Features

The IL2RB Mouse mAb (Aladdin SKU: Ab349821; reference catalog absea#KC-3400) is a monoclonal antibody generated in mouse against the human IL2RB/CD122 protein. Monoclonal antibodies offer a significant advantage over polyclonal preparations in terms of batch-to-batch reproducibility and epitope specificity, both of which are critical for quantitative assays and longitudinal studies.

Key attributes of this reagent include its monospecific recognition of the IL2RB antigen, suitability for use in standard immunological assay formats, and its value as a research tool for dissecting IL-2 and IL-15 receptor biology. Researchers investigating NK cell development, regulatory T cell function, or cytokine-mediated tumor immunology will find this antibody particularly relevant.

Note: Specific performance data including validated applications (e.g., Western blot, flow cytometry, immunofluorescence, ELISA), dilution ranges, and species cross-reactivity should be confirmed from the current product datasheet available on the Aladdin Scientific website or by contacting technical support. Datasheet specifications take precedence over general descriptions.

Research Applications of IL2RB Mouse mAb

IL2RB Mouse mAb is a versatile tool applicable across multiple research contexts within immunology and cell biology. The following areas represent key use cases:

• Lymphocyte Immunophenotyping: CD122 expression is a defining marker for NK cells and memory-phenotype CD8+ T cells. Flow cytometry using IL2RB Mouse mAb enables precise identification and quantification of these populations from peripheral blood or tissue samples.
• Cytokine Signaling Studies: Researchers studying IL-2 or IL-15 receptor complex assembly, receptor internalization, or downstream JAK-STAT signaling can employ this antibody in co-immunoprecipitation, Western blot, or proximity ligation assays.
• Tumor Immunology: Given the role of IL-2 and IL-15 signaling in sustaining cytotoxic lymphocyte activity, IL2RB antibodies are valuable in assessing immune cell infiltration and activation status within the tumor microenvironment.
• Autoimmunity and Immune Dysregulation: Aberrant IL2RB expression is implicated in autoimmune pathologies; this mAb can support expression profiling across patient-derived samples.
• Drug Development Support: As IL2RB is a target of emerging immunotherapeutic strategies, this antibody can serve as a companion reagent in preclinical studies evaluating receptor occupancy or downstream pathway modulation.

Selection and Usage Guidance for Researchers

Selecting the right anti-IL2RB antibody requires careful consideration of the experimental system. Below are practical guidelines for researchers:

Application Compatibility: Confirm that the lot you are ordering has been validated for your specific assay format. The product datasheet for Ab349821 details verified applications; always request the most current datasheet prior to purchase.

Species Reactivity: Verify that the antibody cross-reacts with your experimental model organism (e.g., human, mouse). Cross-reactivity data should be confirmed from the datasheet, as this can vary between lots.

Controls: Include appropriate isotype controls (matched mouse IgG subclass) to distinguish specific from non-specific binding, particularly in flow cytometry and immunofluorescence experiments.

Storage and Handling: Monoclonal antibodies should be stored according to the manufacturer's instructions (typically 2–8 °C for short-term or −20 °C for long-term storage). Avoid repeated freeze-thaw cycles to maintain antibody integrity.

Titration: Optimize antibody concentration empirically for each new cell type, tissue, or assay format. Manufacturer-recommended dilutions serve as starting points only.

Ordering and Technical Support

IL2RB Mouse mAb is available from Aladdin Scientific under SKU Ab349821. To order, visit the Aladdin Scientific product page, add the item to your cart, and proceed through the standard procurement workflow. Bulk or custom order inquiries can be directed to the Aladdin sales team.

For technical questions regarding assay optimization, cross-reactivity, or protocol troubleshooting, contact Aladdin Scientific's technical support team directly. Our scientists can assist with application-specific guidance and provide access to supplementary validation data where available.

Researchers requiring Safety Data Sheets (SDS), certificates of analysis (CoA), or specific lot data should request these documents through the product page or customer service portal. All regulatory and compliance documentation is available upon request.