Is it okay to dilute the rupture agent in intracellular fixation rupture agent with cell staining buffer?

No, it is recommended to use ultrapure, double-distilled, triple-distilled or deionized water for dilution. Dilution of membrane-breaking agents with cell staining buffers can alter the osmotic pressure of the sample itself as well as the salt particle concentration, ultimately leading to poor cell status or even death.

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