Target Overview: What Is LYAR?

LYAR (Ly1 Antibody Reactive homolog) is a zinc finger nucleolar protein that plays important roles in cell growth regulation and ribosome biogenesis. It is primarily localized in the nucleolus and has been associated with the regulation of rDNA transcription, cell proliferation, and apoptosis. LYAR expression has been reported to be dysregulated in certain tumor contexts, making it a subject of interest in cancer biology and cell cycle research.

Research targeting LYAR with specific monoclonal antibodies enables precise detection and characterization of this protein in various experimental settings, supporting studies on nucleolar dynamics, cell growth signaling, and potential disease associations.

Antibody Specifications & Formulation

The LYAR Mouse mAb (Ab350997) is a monoclonal antibody produced in mouse. The product is supplied exclusively in PBS (phosphate-buffered saline) formulation without additional stabilizers or preservatives unless otherwise stated on the Certificate of Analysis. Key specifications include:

• Host Species: Mouse
• Antibody Format: Monoclonal IgG
• Formulation: PBS only
• Supplier Reference: absea#KC-29864
• SKU: Ab350997

Researchers should consult the product's Certificate of Analysis (CoA) for lot-specific details including concentration, purity, and validated applications, as these may vary between lots.

Recommended Protocol & Usage Guidelines

The following general protocol guidelines are provided for researchers using LYAR Mouse mAb. Always refer to the lot-specific datasheet for validated conditions and optimized dilutions.

Storage & Handling

• Store the antibody at –20 °C for long-term storage. Avoid repeated freeze-thaw cycles to preserve antibody integrity.
• For short-term use (up to 1 month), store at 4 °C.
• Keep the product in PBS formulation as supplied; do not dilute until ready for use.

General Application Notes

• Western Blot (WB): An initial dilution range of 1:500–1:2000 is recommended as a starting point; optimize empirically for your specific sample type and detection system.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): Titrate antibody concentration according to cell type and fixation method used.
• Immunoprecipitation (IP): Use recommended amounts per standard IP protocols; confirm specificity with appropriate controls.

Controls & Validation

Always include a positive control (cell line or tissue known to express LYAR) and a negative control (isotype control mouse IgG) in each experiment to confirm specificity and rule out non-specific binding.

Troubleshooting Common Issues

If experimental results are unsatisfactory, consider the following troubleshooting steps:

• No signal detected: Verify that the target protein is expressed in your model system. Check antibody storage conditions and ensure it has not been subjected to repeated freeze-thaw cycles. Confirm secondary antibody compatibility with mouse primary antibody.
• High background: Increase blocking time or adjust blocking agent concentration. Reduce primary antibody concentration. Optimize wash steps to remove unbound antibody.
• Non-specific bands (WB): Increase antibody dilution, extend washing steps, or optimize blocking conditions. Confirm sample preparation integrity.
• Weak signal: Increase primary antibody concentration within the recommended range, extend incubation time, or optimize antigen retrieval conditions if applicable.

If issues persist, contact Aladdin Scientific technical support with lot number, experimental conditions, and results for expert assistance.

Ordering & Quality Assurance

LYAR Mouse mAb is available from Aladdin Scientific under SKU Ab350997. Each lot undergoes quality control testing to ensure consistent performance. A Certificate of Analysis (CoA) is available upon request and provides lot-specific concentration, purity, and validated application data.

For bulk orders, custom formulations, or additional technical documentation, please contact the Aladdin Scientific sales or technical support team. Standard shipping conditions for this antibody should maintain the cold chain; refer to the product datasheet for shipping and handling recommendations specific to this formulation.