Lysate would like to ask what PMSF does with Na3VO4?

Both are enzyme inhibitors that work to prevent protein degradation. PMSF is often used as a protease inhibitor in biochemical and molecular biology experiments. It is an inhibitor of serine proteases and can inhibit the activity of trypsin, chymotrypsin and AChE. Na3VO4 is a phosphatase inhibitor, inhibiting ATPase, alkaline phosphatase and tyrosine phosphatase.