Drosophila has a short life history, high reproduction rate, easy rearing and other characteristics, is a good material for the study of genetics. Through this experiment, students can understand the life history of Drosophila and the morphological characteristics of each stage, the male and female sex characteristics, the characteristics of common mutants, mastering the experimental Drosophila breeding, and experimental treatment methods and techniques, for the preparation of Drosophila hybridization experiments.

Operation method

observation method

Principle

Male flies are smaller, blunt and rounded at the end, dark in color, with 4 ventral segments on the dorsal surface of the abdomen, and a sexual comb at the anterior end of the tarsus of the first pair of legs, whereas females are larger, pointed at the end, lighter in color, and with 5 black stripes on the dorsal surface of the abdomen. Ventral surface with 6 abdominal segments, without sexual comb.

Materials and Instruments

Large larvae
Ether Culture medium
Anesthesia bottle Culture bottle Autoclave

Move

I. Materials and methods
1. Life history observations: egg, larva, pupa, adult. Drosophila life history length and determinants.
2. Methods of anesthesia and observation of traits in Drosophila: anesthesia bottle, ether.
3. Differences between male and female adult flies: dorsal surface of abdomen, body size, shape of abdomen, first pair of forefoot tarsal segments.
4. Morphological characteristics of common mutants of Drosophila: No. 18 (wild type), No. 22 (white eyes), No. 14 (stumpy wings, ebony body), No. 6 (small wings, white eyes, burnt bristles].
5. Drosophila rearing: culture flasks, autoclaves, culture media.
II. Steps
1. Select large larvae and add one or two drops of saline.
2. Staining for 10 minutes.
3. Pressing and observation.

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