There is a great correlation between body temperature and physiological rhythms in mice, and the traditional and simpler method of measuring body temperature in mice is rectal-anal thermometry. This method is widely used because it is simple and easy to prepare. However, the rectal-anal temperature measurement requires a high level of operation from the tester, and the results may fluctuate due to the different depths of the thermometer insertion, so the accuracy of the results is often unsatisfactory. At present, there are 1 main types of temperature measurement experiments for mice: rectal-anal temperature measurement of mouse body temperature.

Operation method

Rectal-anal temperature measurement of body temperature in mice

Materials and Instruments

Equipment:
① Thermometer
② Mice.

Move

The basic process of rectal-anal temperature measurement in mice can be divided into the following steps:

Caveat

1 To ensure comparable results, the assay must be done at the same time each day.2 It should be noted that the standardized values of the assay indexes are different for mice of different ages and strain backgrounds, so for mutant mice of pure background, mice of the same strain background, age and sex, and breeding conditions are required as controls.3 For non-pure background mice, the values of the assayed indexes may differ significantly within the group, and it is necessary to use mice of the same litter and sex as controls.4 At the same time, the quantitative index requires a sufficient number of mice to ensure the reliability of the differences, and generally more than 5 pairs of mice are needed to determine a stable abnormal phenotypic profile.

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