Target Biology: What Is MTHFS?

MTHFS (5-formyltetrahydrofolate cycloligase, EC 6.3.3.2) is an enzyme involved in one-carbon folate metabolism. It catalyzes the ATP-dependent conversion of 5-formyltetrahydrofolate (5-formyl-THF, also known as folinic acid or leucovorin) to 5,10-methenyltetrahydrofolate, thereby regulating the availability of folate cofactors critical for nucleotide biosynthesis, amino acid metabolism, and methylation reactions.

MTHFS is expressed in a variety of mammalian tissues and plays a role in channeling folate derivatives into metabolically active forms. Research into MTHFS is relevant to studies on cell proliferation, cancer metabolism, neural tube defects, and the cellular response to antifolate therapies. Understanding MTHFS expression levels and localization is therefore of significant interest to investigators working in oncology, metabolic disease, and developmental biology.

Antibody Specifications

The MTHFS Mouse mAb (Aladdin Scientific SKU: Ab351014) is a monoclonal antibody raised in mouse against the MTHFS target. Key specifications are summarized below:

• Clonality: Monoclonal
• Host Species: Mouse
• Target: MTHFS (5-formyltetrahydrofolate cycloligase)
• Formulation: Supplied in PBS (Phosphate Buffered Saline)
• Supplier Reference: absea#KC-48925

Monoclonal antibodies offer high specificity and batch-to-batch consistency, making Ab351014 suitable for experiments requiring reproducible and quantitative results. The PBS-only formulation means the antibody contains no carrier proteins or preservatives such as BSA or sodium azide unless otherwise stated, which may be advantageous for certain conjugation or functional assays.

Note: Researchers should consult the product datasheet for complete and up-to-date specifications including concentration, clone number, isotype, and validated applications before use.

Recommended Applications and Usage Guidelines

Monoclonal antibodies targeting MTHFS are commonly employed in the following immunological and biochemical techniques. Researchers are advised to validate the antibody for their specific application and sample type:

• Western Blot (WB): Detection of MTHFS protein in cell or tissue lysates under denaturing conditions. Standard SDS-PAGE followed by membrane transfer and probing with Ab351014 allows assessment of MTHFS molecular weight and relative expression levels.
• Immunohistochemistry (IHC): Localization of MTHFS in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections. Antigen retrieval may be required depending on fixation method.
• Immunofluorescence (IF): Visualization of MTHFS subcellular localization in fixed cell preparations using fluorescently labeled secondary antibodies.
• Immunoprecipitation (IP): Enrichment of MTHFS from complex protein mixtures for downstream analyses.

Important: Always verify validated applications on the official product datasheet. Optimal antibody dilutions and conditions should be determined empirically for each experimental system.

Storage, Handling, and Stability

Proper storage and handling of monoclonal antibodies is essential to maintain activity and ensure reproducible experimental results. The following general guidelines apply to PBS-formulated antibodies such as Ab351014:

• Short-term storage: Store at 4°C for up to 4 weeks if the antibody will be used frequently.
• Long-term storage: Aliquot the antibody to avoid repeated freeze-thaw cycles and store at -20°C or -80°C for extended periods.
• Freeze-thaw cycles: Minimize the number of freeze-thaw cycles, as repeated freezing and thawing can lead to protein aggregation and loss of binding activity.
• Avoid contamination: Use aseptic technique when handling. Do not return unused antibody to the original vial to prevent contamination.
• Carrier-protein-free formulation: As this product is formulated in PBS only, it may require the addition of a stabilizer (e.g., BSA or glycerol) if long-term storage at -20°C is intended, unless otherwise specified by the manufacturer.

Always refer to the product-specific datasheet for storage recommendations confirmed by the manufacturer.

Ordering Information

The MTHFS Mouse mAb is available from Aladdin Scientific. Use the following details when ordering:

• Product Name: MTHFS Mouse mAb
• SKU: Ab351014
• Formulation: PBS Only
• Supplier Reference: absea#KC-48925

For bulk orders, custom formulations, or technical support, please contact the Aladdin Scientific sales or technical support team. Ensure compliance with your institution's biosafety and material transfer policies when ordering research reagents.