Overview of MTREX Mouse mAb

MTREX Mouse mAb (SKU: Ab351015) is a mouse monoclonal antibody raised against the MTREX protein. MTREX (also known as MTREX/MTR4) is an RNA helicase involved in RNA surveillance and degradation pathways within the cell nucleus, playing a critical role in the nuclear RNA exosome targeting complex. This monoclonal antibody is formulated exclusively in PBS (phosphate-buffered saline) without the addition of stabilizers or preservatives, making it suitable for a variety of downstream biochemical and cell biology applications.

The product is referenced under absea catalog number KC-30431 and is distributed by Aladdin Scientific as Ab351015. Researchers working on RNA processing, nuclear surveillance, or exosome-related biology will find this reagent a valuable tool for detecting and studying MTREX protein expression and localization.

Storage, Handling, and Formulation

MTREX Mouse mAb is supplied in PBS only. The following general guidelines apply to ensure optimal antibody performance and longevity:

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot the antibody into single-use volumes and store at −20°C or −80°C to preserve activity and minimize degradation.
• Handling: Thaw on ice prior to use. Gently mix by inversion; do not vortex.
• Buffer: Formulated in PBS (phosphate-buffered saline). If downstream assays require alternative buffers, dialysis or buffer exchange may be performed using standard methods.
• Contamination prevention: Use aseptic technique when aliquoting. Do not return unused antibody to the original vial.

Note: Specific storage temperatures, concentration, and lot-specific information should be confirmed from the product datasheet or Certificate of Analysis (CoA).

Recommended Protocol for Common Applications

While the specific validated applications for MTREX Mouse mAb (Ab351015) should be confirmed via the product datasheet, mouse monoclonal antibodies of this type are commonly employed in the following assays. Optimization of conditions (dilutions, blocking agents, incubation times) is recommended for each experimental system.

Western Blotting (WB)

1. Prepare cell or tissue lysates under standard denaturing conditions (e.g., RIPA buffer with protease inhibitors).
2. Resolve proteins by SDS-PAGE and transfer to PVDF or nitrocellulose membrane.
3. Block membrane with 5% non-fat dry milk or BSA in TBST for 1 hour at room temperature.
4. Incubate with MTREX Mouse mAb at the recommended dilution in blocking buffer overnight at 4°C.
5. Wash 3× with TBST (10 minutes each).
6. Incubate with appropriate HRP-conjugated anti-mouse secondary antibody for 1 hour at room temperature.
7. Detect using chemiluminescent substrate and image.

Immunofluorescence (IF) / Immunocytochemistry (ICC)

1. Fix cells with 4% paraformaldehyde for 15 minutes; permeabilize with 0.1–0.3% Triton X-100.
2. Block with 5% normal serum for 1 hour.
3. Apply MTREX Mouse mAb at recommended dilution; incubate overnight at 4°C.
4. Wash and apply fluorochrome-conjugated anti-mouse secondary antibody.
5. Counterstain nuclei with DAPI; mount and image.

All dilutions and conditions should be empirically optimized. Refer to the product CoA for lot-specific recommendations.

Troubleshooting

If suboptimal results are obtained when using MTREX Mouse mAb, consider the following troubleshooting steps:

For persistent issues, contact Aladdin Scientific technical support with your lot number and experimental details.