This experimental method was obtained from the official website of Chongqing Medical University

Operation method

Observational experiments on the suppression of viable spermatozoa in mouse testes

Principle

Spermatogonia in the testicular seminiferous tubules, under the action of pituitary gonadotropins, undergo 2-3 mitotic divisions, and some of the cells evolve into primary spermatogonia. The primary spermatogonia then undergo two maturation divisions to form spermatocytes, which are immature and undergo complex morphologic changes in the seminiferous tubules before forming tadpole-shaped spermatozoa. After formation, the spermatozoa enter the epididymis, where they finally mature and are stored in the epididymis under the action of epididymal fluid. Therefore, stripping the testis and epididymis and making a cell suspension, many active spermatozoa are visible under the microscope.

Materials and Instruments

Male mice
Saline
Microscope Ophthalmic scissors Dissecting forceps Used needles Dissecting trays Carrier sheets Cover sheets

Move

1. Preparation of live mouse sperm specimens

(1) Neck-breaking method of mouse execution

(2) Cut open the skin and remove the testis and epididymis into a petri dish.

(3) Add a little 37℃ mouse saline, and use ophthalmic scissors to cut the epididymis into tiny particles to make cell suspension.

(4) Take two slides and put 1-2 drops of cell suspension on each slide, and add one drop of a contraceptive drug that inhibits sperm activity on one of the slides as a control group.

(5) Microscopic observation

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