Proteins in the cerebrospinal fluid combine with phenol to form insoluble Pr salts and appear as white turbidity or precipitation. This experiment is derived from the clinical experiment guide of the undergraduate 5-year program of Mudanjiang Medical College.

Operation method

Pandy test

Principle

Proteins in cerebrospinal fluid combine with phenol to form insoluble Pr salts and appear as white turbidity or precipitate.

Materials and Instruments

cerebrospinal fluid (CSF)
Saturated phenol solution

Move

I. Experimental reagents: saturated phenol solution: phenol 10ml plus distilled water 10Oml at 37 ℃ for a few hours, the bottom layer of phenol precipitation, the upper layer of the saturated solution, keep away from light.

Second, the experimental operation: take 2ml of saturated phenol solution in a small test tube, drop CSFI-2 drops in the black tube view immediately observed.

Third, the results of the experiment to determine:

Negative: clear and transparent, does not show fog

Very weak positive: (earth) slightly white fog, can be seen below the star-colored background

Weakly positive: (+) grayish-white cloudy

(+ + +) grayish-white turbid

(+ + + +) white thick flocculent precipitate

(＋＋＋＋) white clots

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