It is a complementary experiment to experimental protocols A and B of the differential display technique (DD) experiment and the gene expression series analysis experiment.

Modern Neuroscience Research Techniques

Author(s): U. Windhorst & H. Johansson Translated by Z. Q. Zhao Jun Chen

Operation method

Patch Flat Labeling Experiment

Materials and Instruments

Solution & Buffer BSA LoTE cDNA Labeling

Move

Experimental program A

1. to two test tubes containing the released cDNA label:

2. Incubate at 37°C for 30 min.

3. Add 150ul LoTE to amplify to 200ul.

4. Extract with an equal volume of PCI as described above and ethanol precipitate (Experimental protocol A, step 2).

5. Dissolve the precipitate in 4ul of LoTE.

1. to the precipitated cDNA label:

2. Incubate at 37℃ for 30 min.

3. Add 170ul of LoTE to amplify to 2004.

4. Extract with an equal volume of PCI as described above and ethanol precipitate (Experimental protocol A, step 2).

5. Dissolve the precipitate in 4ul of LoTE.

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