What Is PDZRN3 and Why Does It Matter?

PDZRN3 (PDZ Domain RING Finger Protein 3) is a member of the RING-type E3 ubiquitin ligase family that contains both a PDZ protein–protein interaction domain and a RING finger domain. These structural features allow PDZRN3 to participate in ubiquitin-mediated protein degradation and scaffold signaling complexes at specific subcellular locations.

Emerging research links PDZRN3 to key developmental and physiological processes including vascular development, neural differentiation, and Wnt signaling regulation. Dysregulation of PDZRN3 expression has been associated with aberrant cell migration and morphogenesis, making it a protein of significant interest in developmental biology and disease research.

Reliable detection reagents such as PDZRN3 Mouse mAb (Ab351027) are therefore essential tools for researchers characterizing PDZRN3 expression patterns, protein interactions, and functional roles in vitro and in vivo.

Product Specifications and Formulation

PDZRN3 Mouse mAb is available from Aladdin Scientific under SKU Ab351027. The antibody is formulated in PBS (phosphate-buffered saline) only, without carrier proteins or preservatives unless otherwise stated on the datasheet. Researchers should confirm the exact concentration, clone identity, and any additives by consulting the official product datasheet prior to use.

Key attributes to verify with the product datasheet include:

• Antibody concentration: Confirm mg/mL before preparing working dilutions.
• Isotype: Typically IgG for mouse monoclonal antibodies; confirm subclass for secondary antibody selection.
• Storage conditions: PBS-formulated antibodies are generally stored at 4 °C for short-term use or aliquoted and stored at −20 °C for long-term stability. Avoid repeated freeze-thaw cycles.
• Expiry: Follow the lot-specific expiry date provided with the vial.

Note: Specific concentration, clone number, and immunogen sequence are not detailed in the available source material and should be confirmed from the official datasheet (absea#KC-50441).

Recommended Applications and Working Dilutions

Monoclonal antibodies against PDZRN3 are commonly employed in a range of immunological and biochemical assays. While specific validated applications for Ab351027 should be confirmed on the official datasheet, the following applications are typical for antibodies of this class:

• Western Blot (WB): Detection of PDZRN3 protein from cell or tissue lysates separated by SDS-PAGE. Typical starting dilutions for mouse monoclonal antibodies range from 1:500 to 1:2000.
• Immunofluorescence (IF): Visualization of PDZRN3 localization in fixed cells or tissue sections. Typical dilutions range from 1:100 to 1:500.
• Immunohistochemistry (IHC): Detection in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Optimization of antigen retrieval conditions is recommended.
• Immunoprecipitation (IP): Enrichment of PDZRN3 and associated protein complexes from lysates.

Important: Always optimize dilutions empirically for your specific sample type, species, and detection system. Consult the product datasheet for validated applications specific to Ab351027.

Sample Preparation and Protocol Guidelines

Optimal results with PDZRN3 Mouse mAb depend on careful sample preparation. The following general guidelines apply to common assay formats:

Cell and Tissue Lysis (for WB and IP)

• Lyse cells or homogenize tissue in an appropriate RIPA or NP-40 lysis buffer supplemented with protease inhibitor cocktail.
• Clarify lysates by centrifugation at 12,000–14,000 × g for 15 minutes at 4 °C.
• Determine protein concentration using BCA or Bradford assay; load 20–50 µg total protein per lane for WB.

Western Blot Protocol (General)

1. Run SDS-PAGE under reducing conditions; transfer to PVDF or nitrocellulose membrane.
2. Block membrane with 5% non-fat dry milk or BSA in TBST for 1 hour at room temperature.
3. Incubate with PDZRN3 Mouse mAb at the recommended dilution overnight at 4 °C.
4. Wash 3× with TBST; incubate with HRP-conjugated anti-mouse IgG secondary antibody for 1 hour at room temperature.
5. Detect signal using ECL substrate and image with a chemiluminescence imaging system.

Storage and Handling of the Antibody

• Upon receipt, centrifuge briefly to collect liquid. Aliquot to avoid repeated freeze-thaw.
• Store working aliquots at 4 °C (use within 1 month); store stock aliquots at −20 °C.
• Do not dilute antibody in advance of immediate use.

Troubleshooting Common Issues

Researchers may encounter the following challenges when using PDZRN3 Mouse mAb. The guidance below is based on general monoclonal antibody best practices:

If problems persist, contact Aladdin Scientific technical support with your lot number and experimental details for personalized assistance.