Under the action of thrombin, fibrinogen releases peptide A and peptide B, and then transforms into fibrin monomer (FM) and fibrin degradation product (FDP) to form a soluble complex, and ichthyosperm sulfate can make FM in the complex free, and then polymerize on its own to present the visible fibrous flocculent or gelatinous jelly, which reflects the presence of FDP (especially the fragmentation of X), and the experiments come from the undergraduate medical school of Mudanjiang. 5-year laboratory guide for laboratory testing majors

Operation method

Plasma ichthyoglobin sulfate paracoagulation time test

Principle

Fibrinogen releases peptide A and peptide B under the action of thrombin, and then transforms into fibrin monomer (FM) and fibrin degradation product (FDP) to form a soluble complex, and ichthyosperm sulfate can make the FM in the complex free, and then polymerize on its own to present the visible fibrous flocculent or gelatin, which reflects the presence of FDP (especially Fragment X).

Materials and Instruments

Sodium citrate solution Cichlidin sulfate solution

Move

I. Experimental reagents:

1. 109 mol/L sodium citrate solution

2. 1O g / L sulfate ichthyoglobin solution (PH6.5) divided into small tubes, placed in a 20 ℃ in the spare

Experimental operation:

1. 0.5 ml of platelet-rich citrate anticoagulated plasma (PPP) was taken into a test tube.

2. Place in a 37℃ water bath for 3 minutes.

3. Add 0.05 ml of 10 g/L ichthyoglobin sulfate solution, mix well, and place in a 37℃ water bath for 15 minutes to observe the results immediately.

III.Judgment of experimental results:

1. Negative: the plasma is clear and unchanged, no insoluble material is produced.

2. positive: plasma in fine or coarse particles precipitate, or fibrin filaments (network) or jelly formation.

Reference value: Negative in normal subjects

Caveat

1. Oxalate, heparin or EDTA salts should not be used as anticoagulants for this test.

2. False-positive results can be caused by careless blood sampling, incomplete anticoagulation, storing the specimen in a refrigerator, and not inspecting the results immediately upon arrival. If the temperature of the water bath is too low or the content of fibrinogen is too low, it will cause false-negative results.

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