Target Biology: Understanding PRSS22

PRSS22 (Serine Protease 22) is a member of the trypsin-like serine protease superfamily, a large and functionally diverse group of enzymes characterized by the conserved catalytic triad of serine, histidine, and aspartate residues. Serine proteases play critical roles in numerous physiological processes including digestion, coagulation, inflammation, and extracellular matrix remodeling.

PRSS22 has been identified as a secreted protease with restricted tissue expression patterns. Research into its biological function and potential roles in disease pathology remains an active area of investigation, making specific, high-quality antibody reagents essential for advancing mechanistic studies.

Researchers studying protease biology, tissue-specific gene expression, or disease-associated protease dysregulation may benefit from utilizing a validated monoclonal antibody against PRSS22 to probe expression levels, localization, and functional activity.

Antibody Format and Formulation

The PRSS22 Mouse mAb (Ab351041) is a mouse-derived monoclonal antibody, providing high specificity and batch-to-batch consistency compared with polyclonal alternatives. Monoclonal antibodies are generated from a single B-cell clone, ensuring that every lot recognizes the same epitope on the PRSS22 target protein.

The antibody is supplied in PBS (phosphate-buffered saline) as the carrier vehicle. This simple formulation — without added carrier proteins such as BSA or stabilizers such as sodium azide — is particularly advantageous for downstream conjugation chemistries, including fluorescent labeling, biotinylation, or coupling to solid supports. Researchers should confirm the absence of any preservative before use in cell-based assays where cytotoxic agents could interfere.

The reference catalog designation from absea (KC-42445) can assist procurement teams in cross-referencing equivalent products during supplier qualification or budget planning.

Recommended Applications and Experimental Considerations

Monoclonal antibodies against serine proteases such as PRSS22 are commonly employed across a range of immunological detection techniques. While specific validated applications for Ab351041 should be confirmed against the manufacturer's datasheet and any available validation data, typical applications for mouse monoclonal antibodies of this class include:

• Western Blotting (WB): Detection of PRSS22 protein in cell lysates or tissue homogenates under denaturing conditions.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative or semi-quantitative detection of PRSS22 in solution-phase samples.
• Immunohistochemistry (IHC): Visualization of PRSS22 distribution in fixed tissue sections.
• Immunofluorescence (IF): Subcellular localization studies in cultured cells or tissue sections.

Researchers should perform titration experiments to determine optimal antibody concentrations for each assay format. Appropriate positive and negative controls, including isotype control antibodies and PRSS22-expressing versus non-expressing cell lines, are strongly recommended to validate specificity.

Storage, Handling, and Stability

Proper storage and handling of monoclonal antibody preparations are critical to maintaining biological activity and assay reproducibility. The following general guidelines apply to PBS-formulated mouse monoclonal antibodies such as Ab351041:

• Short-term storage: Store at 2–8 °C for up to 4 weeks if the antibody will be used frequently.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of binding activity.
• Avoid: Repeated freeze-thaw cycles; exposure to temperatures above 37 °C; storage in frost-free freezers that undergo temperature cycling.
• Carrier-protein-free formulations: Because Ab351041 is supplied in PBS without BSA, the antibody may be more susceptible to adsorption onto tube surfaces at low concentrations. Consider adding a compatible carrier protein (e.g., 0.1% BSA) if working at very low antibody concentrations, unless downstream conjugation is planned.

Always centrifuge briefly before opening the vial to collect any liquid from the cap. Refer to the product-specific Certificate of Analysis (CoA) for lot-specific storage recommendations and expiry information.

Ordering and Procurement Information

PRSS22 Mouse mAb is available from Aladdin Scientific under catalog number Ab351041. Procurement teams can cross-reference this product using the absea reference number KC-42445 for supplier comparison purposes.

When placing an order, laboratory managers should confirm:

• Required quantity and pack size availability.
• Current lot number and associated Certificate of Analysis (CoA) for quality verification.
• Shipping conditions compatible with cold-chain requirements for antibody reagents.
• Any applicable institutional or volume-based pricing promotions.

For technical questions regarding application-specific validation data, recommended dilutions, or compatibility with particular detection systems, contact Aladdin Scientific technical support prior to purchase to ensure Ab351041 meets your experimental requirements.