What Is PTGR2 and Why Does It Matter?

Prostaglandin Reductase 2 (PTGR2) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. It catalyzes the NADPH-dependent reduction of the double bond at the 15-oxo position of prostaglandins and related lipid mediators, playing a key role in the inactivation and metabolism of eicosanoids. Because prostaglandins regulate inflammation, cellular proliferation, and homeostasis, enzymes such as PTGR2 that modulate their activity are of significant interest in cancer biology, metabolic disease, and inflammation research.

Researchers investigating arachidonic acid signaling pathways, lipid mediator metabolism, or prostaglandin-driven disease mechanisms rely on validated reagents such as PTGR2 Mouse mAb (Ab350662) to accurately detect and quantify PTGR2 expression in experimental models.

Product Specifications and Formulation

PTGR2 Mouse mAb (Ab350662) is a monoclonal antibody raised in mouse against the PTGR2 protein. The antibody is supplied in phosphate-buffered saline (PBS) without additional stabilizers or preservatives as currently specified. The reference clone designation is absea #KC-28287, which can assist researchers in cross-referencing published literature or equivalent reagents.

Key specifications:

• Host Species: Mouse
• Antibody Class: Monoclonal (mAb)
• Formulation: PBS only
• Clone Reference: absea #KC-28287
• SKU: Ab350662

For long-term storage, it is generally recommended to aliquot the antibody upon receipt and store at −20°C or below, avoiding repeated freeze-thaw cycles. Specific storage recommendations provided in the product datasheet should always take precedence.

Recommended Applications and Usage Guidelines

Monoclonal antibodies targeting PTGR2 are typically employed in a range of standard immunological assays. While confirmed application data from the product datasheet should be consulted before use, PTGR2 Mouse mAb (Ab350662) is expected to be suitable for common techniques including:

• Western Blotting (WB): Detection of PTGR2 in cell or tissue lysates under denaturing conditions.
• Immunofluorescence (IF): Visualization of PTGR2 subcellular localization in fixed cells.
• Immunohistochemistry (IHC): Detection of PTGR2 expression in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative detection of PTGR2 in solution-phase formats.

Researchers should optimize primary antibody dilutions, incubation times, and antigen retrieval conditions empirically for each assay system and sample type. Consult the official product datasheet for validated dilution ranges and positive control recommendations.

Best Practices for Monoclonal Antibody Handling

To preserve the activity and specificity of PTGR2 Mouse mAb (Ab350662), adhere to the following general best practices:

1. Aliquot on arrival: Divide the antibody into single-use volumes to minimize freeze-thaw cycles, which can degrade performance over time.
2. Cold-chain maintenance: Keep the antibody on ice during experimental setup. Avoid prolonged exposure to room temperature.
3. Avoid azide contamination: Since this product is formulated in PBS only with no stated preservatives, do not add sodium azide if the antibody will be used in cell-based assays, as azide is cytotoxic.
4. Use appropriate blocking: Choose blocking reagents (BSA or non-fat milk) compatible with downstream detection systems and the specific assay format.
5. Include controls: Always include isotype-matched negative controls and validated positive controls to confirm specificity and rule out non-specific binding.