Pyridostatin (RR82): G-Quadruplex Stabilizer FAQs
Pyridostatin (RR82): G-Quadruplex Stabilizer FAQs
Pyridostatin(RR82) is a potent and selective small-molecule G-quadruplex (G4) DNA stabilizer widely used in research to investigate genomic instability, DNA damage responses, and cancer-relevant biological pathways.
- Product Name
- Pyridostatin (RR82)
- SKU
- P128017
- Primary Target
- G-quadruplex (G4) DNA structures
- Key Application
- Induction of genomic instability, DNA damage research, cancer biology
- Supplier
- Aladdin Scientific
- Recommended Storage
- Store as directed on product datasheet; protect from light and moisture
What Is Pyridostatin (RR82)?
Pyridostatin (also known as RR82) is a synthetic small molecule that selectively binds to and stabilizes G-quadruplex (G4) DNA secondary structures. G-quadruplexes are four-stranded nucleic acid structures formed by guanine-rich sequences, found prominently at telomeres, gene promoters, and replication origins throughout the genome. By stabilizing these structures, Pyridostatin interferes with normal DNA replication and transcription processes, making it a valuable chemical tool in research settings.
Pyridostatin is widely used as a research reagent to study DNA damage responses, replication stress, and the biological consequences of G4 stabilization in both normal and cancer cell models.
Mechanism of Action
Pyridostatin functions by binding with high affinity and selectivity to G-quadruplex DNA structures. Upon binding, it stabilizes these non-canonical secondary structures, preventing the unwinding required for normal DNA replication and transcription. This stabilization leads to replication fork stalling, accumulation of DNA double-strand breaks, and activation of the DNA damage response (DDR) machinery, including markers such as γH2AX.
Importantly, Pyridostatin shows preference for G4 structures over duplex DNA, lending it selectivity as a chemical biology probe. Its activity has been demonstrated to promote genomic instability preferentially in cancer cells that rely on specific transcriptional programs driven by G4-containing promoters.
Applications in Research
Pyridostatin (RR82) is employed across a range of research applications:
- DNA Damage Research: Used to induce controlled DNA damage via G4 stabilization, enabling study of cellular repair mechanisms and checkpoint activation.
- Cancer Biology: Investigates the role of G-quadruplex structures in oncogene expression (e.g., MYC, KRAS) and cancer cell proliferation.
- Telomere Biology: Explores the stabilization of telomeric G4 structures and resulting effects on telomerase activity and telomere maintenance.
- Replication Stress Studies: Models replication fork stalling to dissect the molecular pathways of replication stress responses.
- Chemical Biology Probes: Serves as a tool compound for validating G4-targeting strategies in drug discovery pipelines.
Handling, Solubility, and Storage
Proper handling and storage of Pyridostatin (RR82) are essential to maintain reagent integrity. Users should consult the product datasheet provided with SKU P128017 for lot-specific specifications including purity, molecular weight, and recommended storage conditions.
General guidelines (verify with datasheet):
- Store at the temperature specified on the datasheet, typically −20 °C, in a dry environment protected from light.
- Pyridostatin is commonly prepared as a stock solution in DMSO; aqueous solubility may be limited. Always confirm solubility data on the current product datasheet.
- Avoid repeated freeze-thaw cycles; aliquot stock solutions upon first use.
- Handle in accordance with standard laboratory safety practices and consult the Safety Data Sheet (SDS) before use.
Ordering and Technical Support
Pyridostatin (RR82) is available from Aladdin Scientific under SKU P128017. To place an order or request a quote, visit the Aladdin Scientific website or contact our sales team directly.
For technical questions regarding experimental design, dosing, or compatibility with specific assay systems, please reach out to Aladdin Scientific's technical support team. Our specialists can assist with application guidance, solubility recommendations, and protocol optimization to ensure successful outcomes in your research.
Bulk quantities and custom packaging may be available upon request. Please inquire with the procurement or sales team for pricing and availability.
Frequently asked questions
What is the primary mechanism by which Pyridostatin (RR82) exerts its effects?
Pyridostatin selectively binds to and stabilizes G-quadruplex (G4) DNA secondary structures, preventing their resolution during replication and transcription. This leads to replication fork stalling, DNA double-strand break accumulation, and activation of the DNA damage response, including γH2AX formation.
In what solvent is Pyridostatin (RR82) typically dissolved, and are there solubility limitations?
Pyridostatin is commonly prepared as a stock solution in DMSO. Aqueous solubility may be limited, so researchers should verify exact solubility specifications on the current product datasheet for SKU P128017. Avoid introducing excessive DMSO into cell-based assays to prevent cytotoxicity artifacts.
What cell-based research applications is Pyridostatin (RR82) most commonly used for?
Pyridostatin is most commonly used to study DNA damage responses, replication stress, and the transcriptional consequences of G4 stabilization in cancer cell lines. It is also applied in telomere biology research and as a tool compound to validate G-quadruplex-targeting strategies in oncology-focused drug discovery.
How should Pyridostatin (RR82) be stored to maintain its stability?
Pyridostatin should be stored at the temperature specified on the product datasheet (typically −20 °C), in a dry, light-protected environment. To preserve activity, avoid repeated freeze-thaw cycles by preparing single-use aliquots of the stock solution upon first use.
Is Pyridostatin (RR82) selective for G-quadruplex DNA over duplex DNA?
Yes, Pyridostatin demonstrates preferential binding to G-quadruplex DNA structures over conventional double-stranded (duplex) DNA, which underpins its utility as a selective chemical biology tool. However, researchers should consider potential off-target effects at higher concentrations and include appropriate controls in their experimental design.
Can Pyridostatin (RR82) be used in in vivo studies?
Pyridostatin has been reported in the literature as a research tool in cell-based and some in vivo contexts; however, specific in vivo protocols, dosing regimens, and pharmacokinetic data should be verified in the current peer-reviewed literature and are not provided on the reagent datasheet. Consult Aladdin Scientific's technical support for guidance on in vivo suitability.
References
- Rodriguez R, et al. A novel small molecule that alters shelterin integrity and triggers a DNA-damage response at telomeres. J Am Chem Soc. 2008.
- Balasubramanian S, Hurley LH, Neidle S. Targeting G-quadruplexes in gene promoters: a novel anticancer strategy? Nat Rev Drug Discov. 2011.
- Rodriguez R, et al. Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat Chem Biol. 2012.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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