The addition of sodium nitrosoferrocyanide to urine containing ketone bodies resulted in the appearance of a purple ring when it was dissociated from the ammonia solution. This experiment was obtained from the Mudanjiang Medical College undergraduate 5-year clinical experiment guide.

Operation method

Qualitative urine ketone body test

Principle

The addition of sodium nitrosoferricyanide to the ketone body-containing urine was followed by the appearance of a purple ring upon contact with the ammonia solution.

Move

Take about 5ml of fresh urine and put it into a test tube and add about 250mg of sodium nitroso ferrocyanide, then add about 0.5ml of glacial acetic acid and shake it repeatedly to dissolve it, mix it well, add about 2ml of 280g/L NH4OH along the wall of the tube and observe it after static.

III. Judgement of experimental results: Negative No purple ring after 10 minutes.

Trace No purple ring after 10 minutes

(+) Purple ring after 10 minutes

(++) Purple ring appears more quickly.

+ + + + - + + + Immediate purple ring.

Caveat

1. Purple color is obvious when KBT is low, and red color is obvious when KET is high.

2. Ammonia volatilization. Concentration is too low, poor color development.

3. The urine contains a large number of amorphous urate, producing yellow-brown ring.

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