What Is RAB3IL1 and Why Does It Matter?

RAB3IL1, also known as RAB3A Interacting Protein Like 1 or GRAB (guanine nucleotide exchange factor for RAB3A), is a member of the guanine nucleotide exchange factor (GEF) family. It functions by catalyzing the exchange of GDP for GTP on RAB3A, a small GTPase that plays a central role in regulated exocytosis and vesicle trafficking at synaptic terminals and secretory cells.

RAB3 family GTPases are well-established regulators of Ca²⁺-dependent neurotransmitter release and hormone secretion. As a GEF for RAB3A, RAB3IL1 is positioned upstream in the regulatory cascade that controls vesicle docking and fusion at the plasma membrane. Dysregulation of RAB3 signaling has been implicated in neurological disorders and secretory dysfunction, making RAB3IL1 a relevant target for basic research into synaptic physiology and cellular secretion.

Research tools that reliably detect RAB3IL1 are therefore valuable for scientists studying vesicle biology, neuroendocrine function, and GTPase-mediated signaling networks.

Product Specifications of RAB3IL1 Mouse mAb (Ab351048)

The RAB3IL1 Mouse mAb (catalog number Ab351048) from Aladdin Scientific is a mouse-derived monoclonal antibody targeting the RAB3IL1 protein. Key product attributes are summarized below:

• Catalog Number: Ab351048
• Host Species: Mouse
• Antibody Format: Monoclonal Antibody (mAb)
• Formulation: PBS (Phosphate-Buffered Saline) only — no carrier proteins or preservatives stated
• Target: RAB3IL1 (RAB3A Interacting Protein Like 1)

The PBS-only formulation is beneficial for downstream conjugation workflows, functional assays, or when carrier-protein-free conditions are required. Researchers should store the antibody according to the conditions specified on the product datasheet and avoid repeated freeze-thaw cycles to maintain activity.

Note: Specific details including antibody concentration, clone identity, isotype, and validated applications are not fully described in the available source material. Researchers are advised to consult the full product datasheet on the Aladdin Scientific website or contact technical support for complete specifications prior to experimental use.

Recommended Applications and Experimental Considerations

Monoclonal antibodies targeting RAB GTPase pathway components such as RAB3IL1 are commonly employed in a variety of standard immunological and cell biology techniques. While researchers should verify validated applications in the official product datasheet, typical uses for antibodies of this class include:

• Western Blot (WB): Detection of endogenous RAB3IL1 expression across cell lines or tissue lysates, useful for expression profiling and knockdown validation.
• Immunoprecipitation (IP): Isolation of RAB3IL1 protein complexes to study protein-protein interactions within the RAB3 signaling network.
• Immunofluorescence (IF): Subcellular localization studies to assess RAB3IL1 distribution in relation to vesicular compartments.
• Immunohistochemistry (IHC): Tissue-level expression analysis, particularly relevant in neural or neuroendocrine tissues.

When designing experiments with RAB3IL1 Mouse mAb, researchers should optimize primary antibody concentration, select appropriate secondary anti-mouse antibodies, and include relevant positive and negative controls. Since the formulation is PBS only, the antibody may be directly diluted into standard blocking and incubation buffers.

Storage, Handling, and Quality Considerations

Proper storage and handling of monoclonal antibodies are essential to preserving specificity, sensitivity, and batch-to-batch consistency. For RAB3IL1 Mouse mAb (Ab351048), researchers should adhere to the following general best practices for PBS-formulated antibodies:

• Storage Temperature: Short-term storage at 4°C is suitable; for long-term storage, aliquot and store at −20°C or −80°C as indicated on the datasheet.
• Avoid Repeated Freeze-Thaw: Prepare single-use aliquots upon first receipt to minimize freeze-thaw cycles, which can degrade antibody integrity.
• Sterile Handling: Use sterile pipette tips and tubes when handling to prevent microbial contamination of the PBS-only formulation, which lacks preservatives.
• Concentration Optimization: Titrate the antibody in each specific assay format since optimal working dilutions may vary by application and sample type.

Always consult the product-specific datasheet provided by Aladdin Scientific for storage conditions, expiry, and any additional handling instructions specific to lot Ab351048.

Ordering Information and Technical Support

RAB3IL1 Mouse mAb is available from Aladdin Scientific under catalog number Ab351048. Researchers and procurement teams can place orders through the Aladdin Scientific online store or by contacting the regional sales team directly.

For technical inquiries regarding validated applications, recommended dilutions, compatible secondary antibodies, or troubleshooting assistance, Aladdin Scientific's technical support team is available to provide guidance. When contacting support, please have the catalog number (Ab351048) and lot number available to enable accurate and efficient assistance.

Bulk quantities, custom conjugation services, or OEM inquiries may be accommodated upon request — please reach out to your Aladdin Scientific account representative for further information.