The experimental method was obtained from the official website of the Fourth Military Medical University

Operation method

Rat Grabbing Experiment

Materials and Instruments

rats

Move

Rats have sharp teeth, so be careful not to get bitten when catching them. When catching small rats from the cage, grasp the root of the rat's tail and do not let the rat hang in the air for too long, otherwise the rat will be easily provoked and the skin of the tail will easily fall off; when catching large ones, pinch them with the left hand from the center of the back to the chest, but the force should not be too great, and it is better to wear protective gloves, but the gloves should not be too thick (e.g., Fig. 1).

Figure 1

In case of gavage, intraperitoneal injection, intramuscular or subcutaneous injection, the mouse can be manipulated by using the technique of grasping the mouse and adjusting the position of the rat in the hand.

For tail vein blood sampling or injection, the rat can be fixed in a fixator or placed in an inverted beaker according to the mouse tail vein injection method, leaving the tail outside (Figure 2).

Figure 2

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