Product Overview

The Recombinant C9orf78 Antibody (SKU: Ab350229) is a recombinant antibody targeting the C9orf78 protein, a gene product encoded by chromosome 9 open reading frame 78. Recombinant antibodies are produced using defined DNA sequences, ensuring high lot-to-lot consistency and reproducibility compared to traditional hybridoma-derived antibodies. This product is supplied in PBS (phosphate-buffered saline) without additional stabilizers, preservatives, or carrier proteins, making it suitable for a variety of downstream research applications that require minimal buffer interference.

C9orf78 has been reported to play roles in RNA processing and splicing regulation, and accurate detection of this protein is important for studies investigating gene expression, RNA metabolism, and associated cellular pathways. Researchers are advised to consult the latest literature for updated functional insights into C9orf78 biology before designing experiments.

Recommended Applications and Usage Guidelines

Recombinant antibodies supplied in PBS-only formulations are versatile and can be adapted to multiple immunodetection platforms. While specific validated applications for Ab350229 should be confirmed with Aladdin Scientific technical support, typical uses for recombinant antibodies of this class include:

• Western Blot (WB): Dilute the antibody appropriately in blocking buffer (e.g., 5% non-fat milk or BSA in TBST). Optimize primary antibody concentration empirically. Standard incubation is overnight at 4°C.
• Immunofluorescence (IF): Fix and permeabilize cells according to standard protocols. Apply the antibody at the recommended dilution in PBS or an appropriate blocking solution.
• Immunoprecipitation (IP): Use the antibody conjugated or incubated with protein A/G beads. Verify pull-down efficiency by WB.
• ELISA: Can be used as a capture or detection antibody in sandwich or direct ELISA formats, subject to empirical optimization.

Always perform a titration experiment to determine the optimal working concentration for your specific experimental system. Negative and positive controls should be included in all experiments.

Storage, Handling, and Stability

Proper storage and handling of the Recombinant C9orf78 Antibody are critical for maintaining its activity and specificity. Follow these general guidelines for PBS-formulated recombinant antibodies:

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot into single-use volumes and store at −20°C or −80°C. Addition of a carrier protein (e.g., 0.1% BSA) or glycerol (up to 50%) may improve stability during long-term storage; consult Aladdin Scientific for product-specific recommendations.
• Handling: Centrifuge briefly before opening the vial. Work on ice where possible and minimize exposure to light if the antibody is conjugated to a fluorophore.
• Avoid: Detergents, extreme pH, or temperatures above 37°C, all of which can denature the antibody.

Always refer to the product datasheet (absea#RN-14593) for lot-specific storage instructions provided by the manufacturer.

Troubleshooting Common Issues

When working with the Recombinant C9orf78 Antibody, researchers may encounter the following issues. The guidance below reflects general best practices for recombinant antibody troubleshooting:

For persistent issues, contact Aladdin Scientific technical support with your lot number and experimental details.