What Is Recombinant Human Angiopoietin-2 Protein?

Angiopoietin-2 (ANGPT2) is a secreted glycoprotein and a member of the angiopoietin family of growth factors that plays a central role in vascular development and remodeling. It acts primarily as a context-dependent ligand for the Tie2 receptor tyrosine kinase: in the absence of VEGF it can promote endothelial cell apoptosis and vessel regression, whereas in the presence of VEGF it facilitates vascular destabilization and sprouting angiogenesis.

Recombinant Human Angiopoietin-2 Protein (SKU: rp365026) from Aladdin Scientific is a research-grade recombinant form of this protein produced for use in in vitro cell-based assays, signaling pathway studies, and other experimental applications requiring defined, high-purity ANGPT2.

Reconstitution and Storage Guidelines

Reconstitution: Lyophilized recombinant proteins should generally be reconstituted in sterile, ultrapure water or an appropriate carrier buffer (such as PBS containing a carrier protein, e.g., 0.1% BSA) to achieve a working stock concentration. Refer to the specific product datasheet for the recommended reconstitution volume and buffer. Avoid repeated freeze-thaw cycles, as these can reduce protein activity.

Storage: Upon receipt, the lyophilized product should be stored at -20°C or -80°C as indicated on the product datasheet. Once reconstituted, prepare single-use aliquots to minimize freeze-thaw degradation and store at -80°C for long-term stability. Short-term storage at 4°C (up to one week) may be acceptable with a carrier protein, but always consult the product-specific datasheet for authoritative guidance.

Note: Specific storage and reconstitution details for rp365026 should always be confirmed against the current product datasheet.

Research Applications of Angiopoietin-2

Recombinant Human Angiopoietin-2 Protein is widely used in the following research contexts:

• Angiogenesis assays: Studying endothelial cell sprouting, tube formation, and vessel destabilization in vitro.
• Tumor biology: Investigating the role of ANGPT2 in tumor neovascularization and the tumor microenvironment.
• Tie2 receptor signaling: Examining downstream signaling cascades, including PI3K/Akt and MAPK pathways, following Tie2 activation or antagonism.
• Vascular inflammation: Exploring the contribution of ANGPT2 to endothelial activation and inflammatory responses.
• Cell-based bioassays: Used as a stimulant or control reagent in dose-response experiments with endothelial cell lines.

Always validate the protein's bioactivity using an appropriate functional assay before use in critical experiments.

Quality, Purity, and Verification

Research-grade recombinant proteins such as Angiopoietin-2 are typically characterized by the following quality attributes:

• Purity: Assessed by SDS-PAGE and/or HPLC; research-grade proteins commonly achieve ≥90% purity, though the specific purity specification for rp365026 should be confirmed on the product datasheet.
• Biological activity: Verified by a cell-based functional assay (e.g., Tie2 phosphorylation or endothelial cell response assay); the ED50 or specific activity is reported on the datasheet.
• Endotoxin level: Measured by LAL assay; low endotoxin levels are critical for cell-based assays to avoid non-specific inflammatory responses.
• Identity: Confirmed by Western blot or mass spectrometry.

Always request and review the Certificate of Analysis (CoA) for the specific lot of rp365026 received to confirm all quality parameters.

Ordering, Handling, and Customer Support

How to order: Recombinant Human Angiopoietin-2 Protein (rp365026) can be ordered directly through the Aladdin Scientific website or by contacting your local Aladdin Scientific sales representative. Bulk quantities and custom formulations may be available upon request.

Shipping and handling: The product is shipped under conditions appropriate to maintain protein stability (typically on dry ice for frozen products or with appropriate cold packs for lyophilized material). Upon receipt, inspect the package for integrity and store immediately as directed on the datasheet.

Technical support: For questions about experimental design, protocol optimization, or product-specific technical details not addressed in this FAQ, please contact Aladdin Scientific technical support. Our scientific team is available to assist with troubleshooting and application guidance.

Returns and refunds: Please refer to Aladdin Scientific's standard return and refund policy. Products must generally be assessed upon receipt; contact customer service promptly if there is a quality concern.