What Is Annexin A10?

Annexin A10 (ANXA10) is a member of the annexin superfamily, a large group of structurally related proteins characterized by their ability to bind phospholipid membranes in a calcium-dependent manner. The annexin superfamily plays diverse roles in cellular processes including membrane organization, vesicle trafficking, signal transduction, and regulation of ion channel activity.

ANXA10 is encoded by the ANXA10 gene in humans and shares the conserved annexin repeat domain architecture common to all family members. Unlike several other annexins, ANXA10 has a restricted expression pattern and has attracted particular interest as a potential tissue-specific marker and as a protein whose expression is altered in certain malignancies.

The recombinant form of Human Annexin A10 Protein enables researchers to study its biochemical properties, binding partners, and functional roles without dependence on cell or tissue extraction, offering greater consistency and scalability for experimental work.

Biochemical Properties and Structural Features

Like all annexins, Annexin A10 contains multiple annexin repeat units that form a conserved curved disc-shaped structure. This architecture is responsible for coordinating calcium ions and enabling interaction with negatively charged phospholipid membranes, particularly those enriched in phosphatidylserine and phosphatidylinositol.

The calcium-binding endonexin fold within each repeat domain allows conformational changes upon calcium binding, which in turn modulate the protein's affinity for lipid membranes and potential binding partners. This property makes Annexin A10 relevant to studies of membrane-associated signaling events and vesicle biology.

Recombinant production allows the protein to be supplied in defined buffer conditions suitable for downstream biochemical assays, including binding assays, surface plasmon resonance, enzyme-linked immunosorbent assays (ELISA), and Western blot validation experiments.

Research Applications

Recombinant Human Annexin A10 Protein is suitable for a range of in vitro research applications. Key uses include:

• Cancer Biology: ANXA10 expression has been reported to be dysregulated in certain cancers, making the recombinant protein valuable for investigating its role as a potential biomarker or functional contributor to tumor biology. Researchers can use the protein in cell-free assays to characterize molecular interactions relevant to oncology.
• Protein–Protein and Protein–Lipid Interaction Studies: The calcium-dependent membrane-binding activity of ANXA10 can be studied using standard biophysical and biochemical techniques such as co-immunoprecipitation, pull-down assays, and liposome-binding assays.
• Antibody Validation: Recombinant Annexin A10 serves as a reliable positive control antigen for validating ANXA10-specific antibodies in Western blot, ELISA, and immunoprecipitation workflows.
• Structural and Biophysical Studies: The purified recombinant protein can be used in structural biology experiments, including crystallography or cryo-electron microscopy studies aimed at resolving annexin–membrane interaction mechanisms.

Its recombinant origin ensures batch-to-batch reproducibility, which is essential for quantitative assay development and assay standardization.

Handling, Storage, and Quality Considerations

Recombinant proteins require careful handling to maintain activity and integrity. General best practices for Recombinant Human Annexin A10 Protein include:

• Storage: Store according to the manufacturer's recommended conditions, typically at –20°C or –80°C in aliquots to avoid repeated freeze-thaw cycles, which can degrade protein activity and promote aggregation.
• Reconstitution: If supplied in lyophilized form, reconstitute using the recommended buffer (typically sterile PBS or manufacturer-specified buffer) and allow the solution to equilibrate gently. Avoid vortexing.
• Working Concentrations: Determine optimal working concentrations empirically for each assay type. Start with manufacturer-recommended ranges and titrate as needed.
• Quality Control: Recombinant preparations from Aladdin Scientific are subject to quality control testing. Researchers should verify purity by SDS-PAGE and confirm functional activity using relevant assay formats prior to initiating primary experiments.

Maintaining a cold chain during shipping and minimizing freeze-thaw cycles are critical steps for preserving full biological activity of the protein.

Why Choose Aladdin Scientific Recombinant Human Annexin A10 Protein?

Aladdin Scientific supplies Recombinant Human Annexin A10 Protein (SKU: rp365051) to support the needs of modern research laboratories. Key reasons to choose this product include:

• Defined Source and Traceability: Recombinant origin provides a chemically defined, animal-component-free alternative to tissue-extracted preparations, reducing lot-to-lot variability.
• Research-Grade Purity: Manufactured to research-grade specifications suitable for demanding biochemical and cell-free assay applications.
• Reliable Supply: Consistent availability through Aladdin Scientific's established supply network, reducing project delays caused by reagent shortages.
• Technical Support: Aladdin Scientific's technical team is available to assist researchers in optimizing experimental conditions and troubleshooting assay performance.

For procurement inquiries, detailed specifications, and certificate of analysis requests, researchers and lab managers are encouraged to contact Aladdin Scientific directly or visit the product page for SKU rp365051.