What Is ATAD3A and Why Is It Important?

ATAD3A (ATPase Family AAA Domain-Containing Protein 3A) is an integral mitochondrial membrane protein belonging to the AAA-ATPase superfamily. It is ubiquitously expressed in human tissues and localizes predominantly to the inner mitochondrial membrane, where it spans from the inner membrane into the matrix and intermembrane space. ATAD3A plays critical roles in maintaining mitochondrial morphology, regulating mitochondrial DNA (mtDNA) nucleoid organization, controlling mitochondrial dynamics (fusion and fission), and supporting cholesterol metabolism at mitochondrial contact sites. Emerging evidence links ATAD3A dysfunction to neurological disorders and cancer, making it a protein of high translational relevance. Recombinant Human ATAD3A Protein (rp367343) from Aladdin Scientific enables researchers to investigate these functions in controlled in vitro settings.

Product Specifications and Quality

Recombinant Human ATAD3A Protein (SKU: rp367343) is produced as a recombinant protein derived from the human ATAD3A gene. As a research reagent, it is intended for in vitro laboratory use. Researchers should consult the product datasheet supplied with the product or available through Aladdin Scientific's product page for confirmed details on the following parameters, as these may vary by lot:

• Expression system: Please refer to the current product datasheet for the confirmed host expression system (e.g., E. coli, baculovirus/insect cell, or mammalian cell).
• Purity: Typical research-grade recombinant proteins are supplied at >85–95% purity as assessed by SDS-PAGE; confirm exact purity on the Certificate of Analysis (CoA).
• Tag information: The presence and type of affinity tag (e.g., His-tag, GST-tag) should be confirmed on the current datasheet.
• Formulation: Supplied in a stabilizing buffer; exact composition is listed on the product datasheet.
• Molecular weight: Confirm the expected apparent molecular weight by consulting the datasheet, noting that the full-length human ATAD3A protein is approximately 71 kDa; recombinant constructs may differ.

A Certificate of Analysis (CoA) is available for each lot. Contact Aladdin Scientific technical support for lot-specific documentation.

Storage, Handling, and Reconstitution

Proper storage and handling of Recombinant Human ATAD3A Protein are essential to maintain protein integrity and biological activity.

• Short-term storage: Store at 4 °C if the product will be used within 1–2 weeks of receipt.
• Long-term storage: Store at −20 °C or −80 °C for extended periods. Avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Aliquoting: Upon first thaw, it is strongly recommended to aliquot the protein into single-use volumes to minimize freeze-thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the buffer recommended on the product datasheet. Allow the vial to equilibrate to room temperature before opening, and centrifuge briefly before reconstitution.
• Carrier protein: For low-concentration working solutions, consider adding 0.1% BSA (bovine serum albumin) to prevent adsorption to tube surfaces.

Always refer to the specific storage instructions on the product label and datasheet, as formulation details may vary by lot.

Recommended Applications and Experimental Use

Recombinant Human ATAD3A Protein is suitable for a range of in vitro research applications, including but not limited to:

• SDS-PAGE and Western blot: Use as a positive control or for antibody validation in Western blot experiments targeting ATAD3A.
• ATPase activity assays: As a member of the AAA-ATPase family, ATAD3A can be used in in vitro ATPase activity assays to study its enzymatic properties and the effect of inhibitors or mutations.
• Protein–protein interaction studies: Investigate binding partners of ATAD3A in pull-down or co-immunoprecipitation-style in vitro binding assays.
• Structural studies: Suitable as a substrate for biophysical characterization techniques.
• Drug screening: Use in biochemical screens to identify small molecules that modulate ATAD3A ATPase activity.

This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use. Optimal working concentrations should be determined empirically for each specific assay format.

Ordering, Shipping, and Technical Support

Recombinant Human ATAD3A Protein (rp367343) is available directly from Aladdin Scientific. Below is practical guidance for procurement and support:

• Ordering: The product can be ordered via the Aladdin Scientific website using SKU rp367343, or by contacting your regional Aladdin Scientific sales representative.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Please inspect the product upon receipt and confirm that it arrived in frozen or appropriately cold condition. Report any discrepancies to Aladdin Scientific customer service promptly.
• Returns and replacements: If the product does not meet the specifications stated on the CoA, contact Aladdin Scientific customer service to initiate a return or replacement request in accordance with the company's returns policy.
• Technical support: For questions about experimental design, reconstitution, or troubleshooting, contact Aladdin Scientific technical support with your lot number and CoA at hand.
• Documentation: Safety Data Sheet (SDS), Certificate of Analysis (CoA), and product datasheet are available upon request or via the Aladdin Scientific website.