What Is ATL3 and Why Is It Scientifically Important?

ATL3 (Atlastin GTPase 3, also known as ATL3 or Brain-specific Angiogenesis Inhibitor 1-Associated Protein 2-Like Protein 2) is a member of the atlastin subfamily of dynamin-related GTPases. It is an integral membrane protein predominantly localized to the endoplasmic reticulum (ER), where it plays a critical role in driving homotypic membrane fusion to establish and maintain the characteristic tubular ER network.

The atlastin family—comprising ATL1, ATL2, and ATL3—are large GTPases that catalyze GTP hydrolysis to power membrane tethering and fusion events. ATL3 is expressed ubiquitously but is notably enriched in neurons, making it particularly relevant to peripheral nervous system integrity. Loss-of-function or dominant-negative mutations in ATL3 disrupt ER morphology, leading to fragmented or collapsed ER tubule networks in affected cells.

From a disease perspective, point mutations in ATL3 have been identified in patients with Hereditary Sensory and Autonomic Neuropathy type I (HSAN-I) and overlap with pathways implicated in Hereditary Spastic Paraplegia (HSP). These connections make ATL3 an important target for mechanistic studies in neurodegeneration, axonal maintenance, and ER stress research.

Recombinant Human ATL3 Protein: Product Overview

Aladdin Scientific offers Recombinant Human ATL3 Protein under SKU rp366109, sourced in reference to absea catalog entry PP-1752. This recombinant protein is produced for research use and is intended to support a range of in vitro biochemical and cell biology experiments.

Typical research applications for recombinant ATL3 protein include:

• Biochemical assays: GTPase activity measurements, membrane tethering assays, and liposome fusion reconstitution experiments.
• Binding studies: Investigation of ATL3 interactions with other ER-shaping proteins such as reticulons, DP1/Yop1, or lunapark.
• Structural studies: Use as a reference standard or in biophysical analyses including SPR and ITC.
• Antibody validation: As a positive control antigen for Western blot or ELISA development.

Researchers should consult the product datasheet for confirmed expression system, tag information, purity grade, molecular weight, endotoxin levels, and recommended storage conditions specific to the current lot, as these specifications may vary.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of recombinant proteins are critical to preserving activity and ensuring reproducible experimental results. While lot-specific conditions are detailed on the product datasheet (which takes precedence), the following represent general best-practice guidelines for recombinant GTPase proteins such as ATL3:

• Short-term storage (≤1 week): Store at 4 °C in the supplied buffer.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C to minimize freeze-thaw cycles. Repeated freeze-thaw cycles can lead to protein aggregation and loss of GTPase activity.
• Reconstitution: If supplied lyophilized, reconstitute in sterile ultrapure water or the recommended buffer to the suggested concentration. Allow the vial to equilibrate to room temperature before opening, then mix gently—do not vortex.
• Carrier protein: For dilute working solutions, consider adding a carrier protein (e.g., 0.1% BSA) to reduce adsorptive losses to tube surfaces.
• Avoid repeated freeze-thaw: Prepare single-use aliquots immediately after reconstitution.

Note: Always refer to the current lot-specific Certificate of Analysis (CoA) and datasheet supplied with the product for authoritative storage and handling instructions.

Ordering, Shipping, and Regulatory Information

Recombinant Human ATL3 Protein (rp366109) is available for order directly through Aladdin Scientific. The following information is intended to assist lab managers and procurement personnel:

• How to order: Place orders via the Aladdin Scientific website, by email, or through your institutional procurement system. Reference SKU rp366109 when ordering to ensure accurate fulfillment.
• Shipping conditions: Recombinant proteins are typically shipped on dry ice (−80 °C) or with cold packs depending on formulation (lyophilized vs. liquid). Specific shipping conditions will be confirmed at the time of order processing.
• Intended use: This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or veterinary use.
• Export compliance: Customers are responsible for ensuring that the import and use of this product complies with all applicable local laws and regulations.
• Returns and refunds: Please contact Aladdin Scientific customer service within the specified return window if the product does not meet the stated specifications. Refer to the Aladdin Scientific returns policy for full details.

For pricing, availability, bulk quantity inquiries, or custom formulation requests, contact Aladdin Scientific customer support directly.