Biological Background of ATP1B1

ATP1B1 encodes the beta-1 subunit of the Na+/K+-ATPase, a ubiquitous plasma membrane enzyme that actively transports sodium and potassium ions across the cell membrane. This electrogenic pump maintains the electrochemical gradients essential for cellular excitability, secondary active transport, and osmotic balance.

The beta subunit, encoded by ATP1B1, plays a regulatory role in the Na+/K+-ATPase complex. It is required for the correct folding, membrane insertion, and functional maturation of the catalytic alpha subunit. Beyond structural support, ATP1B1 participates in cell adhesion and has been implicated in diverse physiological processes including neuronal activity, cardiac function, and renal ion handling.

Dysregulation of ATP1B1 expression has been associated with pathological states such as hypertension, cardiac hypertrophy, and certain cancers, making it a target of sustained scientific interest.

Protein Characteristics and Production

Recombinant Human ATP1B1 Protein (SKU: rp364710) is produced using recombinant expression systems to yield a protein suitable for use in biochemical and cell-based assays. Recombinant production enables consistent batch-to-batch quality, well-defined purity, and scalable supply for research applications.

The beta-1 subunit is a type II transmembrane glycoprotein under native conditions. Recombinant formats may vary in glycosylation status depending on the expression host employed (e.g., mammalian, insect, or bacterial systems). Researchers should consult the product datasheet for confirmed expression host, molecular weight, tag information, purity grade, and formulation details specific to SKU rp364710.

Typical quality control assessments for recombinant proteins of this class include SDS-PAGE for purity confirmation and functional assays or ELISA to verify biological activity. Specific QC data should be verified on the official product datasheet.

Research Applications

Recombinant Human ATP1B1 Protein is suited for a range of research applications focused on ion transport physiology, enzyme biochemistry, and disease mechanism investigation. Key applications include:

• Protein–protein interaction studies: ATP1B1 can be used as bait or prey in pull-down assays to map interaction partners within the Na+/K+-ATPase complex or associated signaling networks.
• Antibody validation and immunoassay development: The recombinant protein serves as a positive control antigen for ELISA, Western blotting, and antibody specificity testing.
• Structural and functional studies: Suitable for in vitro binding assays, activity reconstitution experiments, and structural characterization efforts.
• Drug discovery and target validation: Useful for screening small-molecule modulators of Na+/K+-ATPase activity and evaluating compound interactions with the beta subunit.
• Cell biology research: Can be applied in studies exploring cell adhesion, migration, and signaling events mediated through ATP1B1.

Researchers should validate the protein's suitability for each specific application by consulting the product datasheet and conducting appropriate pilot experiments.

Handling, Storage, and Reconstitution

Proper handling of Recombinant Human ATP1B1 Protein is essential to maintain protein integrity and experimental reproducibility. The following general guidelines apply to most recombinant proteins of this type; always defer to the specific instructions provided in the product datasheet for SKU rp364710:

• Storage temperature: Long-term storage at -80°C is generally recommended for recombinant proteins. Short-term storage at -20°C may be acceptable depending on formulation.
• Freeze-thaw cycles: Minimize freeze-thaw cycles to prevent protein aggregation and loss of activity. Prepare single-use aliquots upon first thaw.
• Reconstitution: If supplied as a lyophilized powder, reconstitute in the recommended buffer as specified on the datasheet. Allow the vial to equilibrate to room temperature before opening.
• Carrier protein: Addition of a carrier protein such as BSA (0.1–1% w/v) to the working solution may improve protein stability during dilution and storage.
• Avoid repeated pipetting: Gentle mixing is preferred; avoid vortexing to minimize denaturation.

All storage and handling details must be confirmed against the official product datasheet, as formulation specifics may vary by lot.

Quality, Sourcing, and Ordering Information

Aladdin Scientific offers Recombinant Human ATP1B1 Protein under SKU rp364710, referenced as absea#PP-087. This product is intended for research use only and is not approved for diagnostic or therapeutic applications.

When placing an order or evaluating this reagent for your laboratory, consider the following:

• Purity and QC: Confirm purity grade and quality control data by reviewing the Certificate of Analysis (CoA) available for each lot.
• Quantity options: Contact Aladdin Scientific or visit the product page for available size options and bulk pricing.
• Regulatory compliance: This product is for research use only (RUO). Ensure compliance with institutional biosafety and ethical guidelines for handling recombinant proteins.
• Technical support: Aladdin Scientific's technical team is available to assist with application-specific questions, protocol optimization, and troubleshooting.

For complete specifications, pricing, and availability, visit the Aladdin Scientific website or contact customer service directly.