Overview of ATP8B2 and Its Biological Role

ATP8B2 (ATPase Phospholipid Transporting 8B2) is a member of the P4-ATPase subfamily, a group of transmembrane proteins that function as phospholipid flippases. These enzymes catalyze the ATP-dependent translocation of specific phospholipids from the outer to the inner leaflet of lipid bilayers, thereby maintaining membrane asymmetry — a critical feature of eukaryotic cell biology.

The ATP8B2 protein is encoded by the ATP8B2 gene and is expressed in various human tissues. Like other P4-ATPases, it is thought to interact with CDC50 family accessory subunits, which are required for its proper folding, trafficking, and catalytic activity at the plasma membrane or intracellular compartments.

Research into ATP8B2 contributes to the broader understanding of lipid homeostasis, membrane biology, and diseases associated with defective phospholipid transport, including hepatic and neurological disorders linked to related P4-ATPase family members.

Reagent Specifications and Quality Control

Recombinant Human ATP8B2 Protein (SKU: rp366537) is produced under controlled expression conditions to ensure consistency and reliability across experiments. Researchers should consult the accompanying product datasheet for lot-specific information including host expression system, tag configuration, molecular weight, purity grade, and endotoxin levels.

Prior to use, it is recommended to verify reagent integrity using the following quality control measures:

• SDS-PAGE: Confirm expected molecular weight band and assess purity. A single predominant band indicates high-purity preparation.
• Western Blot: Use a validated anti-ATP8B2 antibody to confirm protein identity.
• Protein Concentration: Determine using a BCA or Bradford assay calibrated with BSA standards.
• Activity Assay (where applicable): ATPase activity can be assessed using inorganic phosphate release assays, subject to the active form of the protein supplied.

Please note that specific purity percentages, exact molecular weight values, and expression system details are lot-dependent and should be taken from the official Certificate of Analysis (CoA) provided by Aladdin Scientific.

Storage, Reconstitution, and Handling Guidelines

Proper handling of Recombinant Human ATP8B2 Protein is essential to preserve biological activity and avoid degradation. The following general guidelines apply to recombinant proteins of this class; always refer to the product-specific datasheet for authoritative instructions:

• Short-term storage (≤1 week): Store at 4°C in the supplied buffer. Do not freeze and thaw repeatedly.
• Long-term storage: Aliquot into single-use volumes and store at -80°C. Record aliquot dates.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended volume of sterile ultrapure water or the buffer specified in the datasheet. Allow the vial to equilibrate to room temperature before opening. Mix gently by pipetting; do not vortex.
• Carrier protein: For low-concentration working solutions, consider adding 0.1% BSA (bovine serum albumin) to stabilize the protein during dilution.
• Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and detergents unless specifically required by the assay design.

Membrane-associated P4-ATPases may require detergent-based solubilization conditions. Consult the datasheet and relevant literature for guidance specific to transmembrane protein handling.

Experimental Applications and Assay Recommendations

Recombinant Human ATP8B2 Protein is suitable for a range of in vitro research applications. Researchers should validate reagent performance in their specific experimental context. Typical applications include:

• Biochemical Characterization: Study ATP hydrolysis kinetics, substrate specificity, and inhibitor screening for P4-ATPase family members.
• Lipid Flippase Activity Assays: Fluorescently labeled phospholipid analogs (e.g., NBD-labeled phosphatidylserine or phosphatidylcholine) can be used in liposome-based assays to assess flippase activity.
• Protein–Protein Interaction Studies: Investigate interactions between ATP8B2 and CDC50 family chaperones (e.g., CDC50A/TMEM30A) using co-immunoprecipitation, pull-down assays, or surface plasmon resonance.
• Antibody Validation: Use as a positive control antigen in Western blot, ELISA, or immunoprecipitation to validate anti-ATP8B2 antibodies.
• Structural Studies: As a substrate for cryo-EM or X-ray crystallography studies investigating P4-ATPase architecture and conformational states.

Optimal working concentrations and assay conditions should be determined empirically. Pilot titration experiments are strongly recommended before large-scale studies.

Safety, Compliance, and Ordering Information

Recombinant Human ATP8B2 Protein is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or veterinary use. Users are responsible for complying with all applicable institutional biosafety guidelines when handling recombinant proteins derived from human sources.

Safety considerations:

• Handle in accordance with your institution's biosafety protocols for BSL-1 recombinant protein materials.
• Wear appropriate personal protective equipment (PPE): lab coat, gloves, and eye protection.
• Dispose of protein solutions and contaminated materials in compliance with institutional and local regulations.
• Consult the Safety Data Sheet (SDS) provided by Aladdin Scientific for detailed hazard and disposal information.

Ordering: Recombinant Human ATP8B2 Protein is available from Aladdin Scientific under SKU rp366537. For volume pricing, custom formulations, or technical support, contact Aladdin Scientific's customer service team. Procurement teams are encouraged to reference the CoA and SDS when completing institutional purchasing documentation.