What Is ATP9A and Why Is It Scientifically Important?

ATP9A (ATPase Phospholipid Transporting 9A) is a member of the P4-ATPase subfamily, also known as type IV P-type ATPases or phospholipid flippases. P4-ATPases are evolutionarily conserved transmembrane enzymes responsible for maintaining the asymmetric distribution of phospholipids between the two leaflets of biological membranes. This asymmetry is critical for numerous cellular processes, including vesicle biogenesis, membrane curvature, cell signaling, and apoptosis.

ATP9A is thought to function as a phospholipid translocase, catalyzing the movement of specific phospholipid species from the exoplasmic (outer) leaflet to the cytoplasmic (inner) leaflet of intracellular membranes. Research indicates ATP9A localizes to endosomes and the trans-Golgi network, where it may play roles in endosomal sorting and recycling pathways.

Note: Detailed mechanistic data for ATP9A remain an active area of investigation; researchers should consult the current primary literature for the most up-to-date findings.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human ATP9A Protein (SKU: rp367920) is produced to research-grade standards. The product is referenced under supplier code absea#PP-3141. Researchers should consult the current product datasheet for the most current and verified specifications, including:

• Expression System: Please refer to the product datasheet for the confirmed expression host (e.g., mammalian, insect, or E. coli system).
• Purity: Purity grade and method of determination (e.g., SDS-PAGE, HPLC) are detailed on the product datasheet.
• Formulation and Buffer: Refer to the datasheet for buffer composition, carrier proteins, and concentration.
• Molecular Weight: Refer to the datasheet for confirmed apparent and predicted molecular weights.

All specifications should be verified against the current lot-specific Certificate of Analysis (CoA) prior to experimental use.

Recommended Research Applications

Recombinant Human ATP9A Protein is suitable for a range of in vitro research applications. Typical uses for recombinant P4-ATPase family members include:

• Biochemical Assays: Enzyme activity studies, ATPase activity assays, and substrate specificity characterization.
• Protein–Protein Interaction Studies: Pull-down assays, co-immunoprecipitation, and yeast two-hybrid validation experiments.
• Antibody Development and Validation: Use as a positive control antigen in ELISA and Western blotting.
• Structural Biology: Preliminary biophysical characterization and crystallization trials (subject to the purity and formulation confirmed on the datasheet).
• Cell Biology Research: Studies of endosomal membrane dynamics, phospholipid asymmetry, and vesicular trafficking pathways.

Researchers should validate the protein for each specific application. Application suitability should be confirmed on the current product datasheet.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human ATP9A Protein are essential to maintain activity and integrity. General best-practice guidelines for recombinant proteins of this class include:

• Storage Temperature: Refer to the product datasheet for the recommended storage temperature. Most recombinant proteins are stored at −20 °C or −80 °C for long-term stability.
• Avoid Repeated Freeze–Thaw Cycles: Aliquot the protein upon first thaw to minimize degradation and loss of activity caused by repeated freeze–thaw cycles.
• Working Concentration: Dilute only the amount required for immediate use; keep unused aliquots frozen.
• Carrier Protein: If the formulation includes BSA or glycerol as a stabilizer, account for this in downstream assay design.
• Expiry and Lot Number: Always record the lot number and check the expiry date before use; refer to the CoA for lot-specific stability data.

Always consult the lot-specific datasheet for definitive storage and handling instructions.

Ordering and Technical Support

Recombinant Human ATP9A Protein is available from Aladdin Scientific under SKU rp367920 (supplier reference absea#PP-3141). To place an order or request a quotation, contact your regional Aladdin Scientific representative or visit the Aladdin Scientific website.

For technical questions regarding experimental design, recommended assay conditions, or troubleshooting, please contact Aladdin Scientific's technical support team. When reaching out, have the following information ready:

• Product SKU and lot number
• Intended application and experimental conditions
• Any observed issues or unexpected results

For questions about pricing, promotions, shipping, returns, or account management, please refer to the relevant sections of the Aladdin Scientific Help Center or contact customer service directly.