What Is ATPAF1 and Why Does It Matter in Research?

ATPAF1 (ATP Synthase Mitochondrial F1 Complex Assembly Factor 1) is a nuclear-encoded protein that localizes to mitochondria and functions as a dedicated chaperone for the assembly of the F1 sector of mitochondrial ATP synthase (Complex V). Complex V is the terminal enzyme of the oxidative phosphorylation (OXPHOS) system, responsible for synthesizing the majority of cellular ATP from ADP and inorganic phosphate, driven by the proton gradient across the inner mitochondrial membrane.

ATPAF1 is specifically involved in the early assembly steps of the F1 catalytic core, facilitating the proper folding and incorporation of the alpha and beta subunits. Disruption of ATPAF1 function has been linked to mitochondrial disease phenotypes characterized by Complex V deficiency, underscoring its physiological importance. Researchers studying mitochondrial biogenesis, OXPHOS disorders, and metabolic diseases find ATPAF1 an important target for mechanistic investigation.

Product Specifications and Quality Attributes

The Recombinant Human ATPAF1 Protein available from Aladdin Scientific (SKU: rp373963; supplier reference absea#PP-7805) is produced using recombinant expression technology. Key specification details—including the exact host expression system, tag configuration, molecular weight on SDS-PAGE, purity percentage, formulation buffer, and endotoxin level—should be confirmed directly from the product datasheet available on the Aladdin Scientific product page or by contacting technical support, as these parameters are subject to lot-specific quality control.

General expectations for a research-grade recombinant human protein of this type include: recombinant expression in a suitable host (e.g., E. coli, baculovirus/insect cells, or mammalian cells), purification to >85% purity as assessed by SDS-PAGE or HPLC, and quality control by standard biochemical methods. Always consult the current Certificate of Analysis (CoA) for lot-specific data before use.

Storage, Handling, and Stability Recommendations

Recombinant proteins generally require careful handling to maintain activity and integrity. The following are standard best-practice recommendations applicable to recombinant human proteins such as ATPAF1:

• Short-term storage (≤1 week): Store at 4°C in the supplied formulation buffer.
• Long-term storage: Aliquot into single-use volumes and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and detergent-containing buffers unless specified.
• Carrier protein: For highly dilute solutions, addition of a carrier protein (e.g., 0.1% BSA) may help prevent adsorptive losses.

Always refer to the product-specific datasheet and CoA for validated storage conditions and shelf-life data for each lot of rp373963.

Research Applications of Recombinant Human ATPAF1 Protein

Recombinant Human ATPAF1 Protein is suitable for a range of in vitro and cell-free research applications, including but not limited to:

• Protein–protein interaction studies: Investigating binding partners within the ATP synthase assembly pathway using co-immunoprecipitation, pull-down assays, or surface plasmon resonance (SPR).
• Antibody validation: Serving as a positive control antigen for western blotting or ELISA to validate anti-ATPAF1 antibodies.
• Enzyme and assembly assays: Functional reconstitution experiments examining the role of ATPAF1 in F1 subunit assembly in vitro.
• Structural biology: As a protein standard or component in cryo-EM or X-ray crystallography studies of Complex V assembly intermediates.
• Biomarker and disease research: Supporting studies of mitochondrial Complex V deficiency and related rare metabolic disorders.

Researchers should optimize assay conditions (protein concentration, buffer composition, temperature) empirically for each specific application.

Ordering and Technical Support Information

Recombinant Human ATPAF1 Protein is available exclusively through Aladdin Scientific under SKU rp373963. To place an order or request a quotation, visit the product page on the Aladdin Scientific website or contact your regional sales representative.

For technical inquiries—including questions about compatibility, experimental design, or lot-specific data—please reach out to the Aladdin Scientific technical support team. When contacting support, have the following information ready: SKU (rp373963), lot number (found on the product vial label and CoA), and a brief description of your intended application.

Bulk quantities, custom formulations, or OEM arrangements may be available upon request. All products are subject to Aladdin Scientific's standard terms and conditions, including applicable return, refund, and shipping policies.