What Is AZI2 and Why Is It Important in Research?

AZI2, also known as 5-Azacytidine-Induced Protein 2, NAP1, or SINTBAD, is a scaffold adaptor protein that plays a central role in the innate immune response. It functions by facilitating the activation of TANK-binding kinase 1 (TBK1) and IκB kinase ε (IKKε), two kinases essential for the induction of type I interferons upon viral infection.

AZI2 is part of a larger signaling complex that links pattern recognition receptor pathways—such as those activated by RIG-I and cGAS-STING—to downstream transcription factors including IRF3 and IRF7, which drive interferon gene expression. Disruption or modulation of AZI2 function can significantly alter the magnitude of the antiviral interferon response, making it a valuable research target in virology, immunology, and inflammatory disease studies.

Recombinant Human AZI2 Protein enables researchers to perform in vitro binding assays, enzymatic activity studies involving TBK1 complex formation, and structural investigations without relying on endogenous protein expression from cell lysates.

Product Specifications and Quality Attributes

Recombinant Human AZI2 Protein (SKU: rp364996) is produced as a recombinant protein intended for research applications. The product is referenced under supplier code absea#PP-393. Key quality attributes that researchers should verify upon receipt include:

• Purity: Recombinant proteins are typically characterized by SDS-PAGE and may be further validated by Western blot. Researchers should consult the Certificate of Analysis (CoA) supplied with each lot for confirmed purity levels.
• Formulation: Recombinant proteins are generally supplied in a buffered solution; the specific buffer composition, protein concentration, and presence of carrier proteins or stabilizers should be confirmed in the product datasheet.
• Endotoxin levels: For applications sensitive to LPS contamination, endotoxin content should be reviewed in the lot-specific CoA.
• Molecular weight: The predicted molecular weight of AZI2 should correspond to the full-length or truncated construct used; consult the datasheet for the expressed region.

All specifications are lot-dependent. Aladdin Scientific recommends reviewing the CoA and product datasheet before experimental use.

Storage, Handling, and Stability Recommendations

Proper storage and handling of Recombinant Human AZI2 Protein are essential to maintain activity and integrity throughout the product's shelf life. General best-practice guidelines for recombinant proteins of this type include:

• Short-term storage (≤1 month): Store at 4 °C in the supplied buffer. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Long-term storage: Aliquot the protein upon receipt and store at −20 °C or −80 °C. Single-use aliquots help preserve activity.
• Handling: Keep the protein on ice during experimental setup. Minimize exposure to protease-containing environments by using appropriate protease inhibitors when preparing working solutions.
• Reconstitution: If supplied lyophilized, reconstitute in the recommended buffer as specified in the product datasheet. Allow to equilibrate gently; avoid vortexing.

Note: Specific storage conditions for this lot should always be confirmed against the product datasheet and CoA provided by Aladdin Scientific.

Recommended Applications for Recombinant Human AZI2 Protein

Recombinant Human AZI2 Protein is suitable for a range of in vitro research applications. Researchers in innate immunity, antiviral biology, and signal transduction commonly use recombinant AZI2 for:

• Protein–protein interaction studies: AZI2 interacts with TBK1, IKKε, TANK, and TBKBP1. Recombinant AZI2 can be used in co-immunoprecipitation assays, pull-down assays, or surface plasmon resonance (SPR) experiments to map binding interfaces.
• Kinase complex reconstitution: In vitro reconstitution of the TBK1 signaling complex using recombinant components allows mechanistic dissection of kinase activation and substrate phosphorylation.
• Antibody validation: Recombinant AZI2 serves as a positive control antigen for validating anti-AZI2 antibodies in Western blot, ELISA, and dot-blot formats.
• Structural biology: Pure recombinant AZI2 preparations can support X-ray crystallography, cryo-electron microscopy, or NMR studies aimed at resolving AZI2 domain architecture.
• Drug screening: AZI2 is a potential target for modulating innate immune responses; recombinant protein can be used in binding or competition assays to screen small-molecule inhibitors.

Researchers should verify that the expressed region and tag configuration of the supplied protein are compatible with their intended assay format.

Ordering, Documentation, and Support

Recombinant Human AZI2 Protein is available from Aladdin Scientific under SKU rp364996. To place an order or request additional technical documentation:

• Online ordering: Visit the Aladdin Scientific website and search by SKU rp364996 or product name to add the item to your cart. Institutional purchase orders are accepted.
• Certificate of Analysis (CoA): Lot-specific CoA documents can be requested through the product page or by contacting customer support. The CoA contains confirmed purity, concentration, and quality control data.
• Safety Data Sheet (SDS): An SDS is available upon request and provides safe handling, storage, and disposal information in compliance with regulatory requirements.
• Technical support: For application-specific questions, protocol assistance, or custom requirements, contact Aladdin Scientific's technical support team.
• Returns and refunds: Aladdin Scientific's returns policy applies; please review terms before purchase, particularly for temperature-sensitive products.

Reference supplier code absea#PP-393 when communicating with suppliers or cross-referencing catalog information from partner distributors.