Introduction to C4orf17 and Its Biological Context

C4orf17, or Chromosome 4 Open Reading Frame 17, is a protein-coding gene located on human chromosome 4. Like many open reading frame (ORF) proteins, C4orf17 has been identified through genome sequencing projects, and its precise biological function remains an active area of investigation in the research community. ORF proteins frequently represent understudied targets with significant potential for uncovering novel cellular pathways.

As genomic and proteomic technologies advance, researchers increasingly require access to purified, recombinant forms of less-characterized proteins such as C4orf17 to facilitate functional characterization, interactome mapping, and structural studies. Recombinant Human C4orf17 Protein provides a defined reagent to support these exploratory research efforts.

Protein Production and Quality Specifications

Recombinant Human C4orf17 Protein (SKU: rp375803) is produced using established recombinant expression systems. Recombinant proteins of this type are typically expressed in suitable host systems such as E. coli, baculovirus-infected insect cells, or mammalian expression platforms, followed by affinity chromatography purification to achieve research-grade purity.

Quality control for recombinant proteins of this class typically encompasses SDS-PAGE analysis to confirm apparent molecular weight, and purity assessment. Researchers should consult the product datasheet for lot-specific certificate of analysis (CoA) data, including purity grade, formulation buffer, concentration, and recommended storage conditions, to ensure optimal experimental performance.

Note: Specific purity percentage, host expression system, and molecular weight data for this product should be verified against the current product datasheet or CoA, as specifications may vary by lot.

Research Applications

Recombinant Human C4orf17 Protein is suitable for a range of in vitro research applications. Typical uses for recombinant ORF proteins include:

• Protein–protein interaction studies: Pull-down assays and co-immunoprecipitation experiments to identify binding partners and map interaction networks.
• Antibody validation and immunoassay development: As a positive control antigen for ELISA, Western blot, and immunofluorescence assay development.
• Biochemical activity assays: Where enzymatic or binding activity has been postulated, recombinant protein enables direct functional testing.
• Structural biology: As starting material for crystallography or cryo-EM studies to elucidate three-dimensional structure.

Researchers are advised to validate the protein's performance in their specific assay system before committing to large-scale experiments, and to consult the current datasheet for recommended working concentrations and buffer compatibility.

Handling, Storage, and Reconstitution Guidelines

Proper handling of recombinant proteins is essential to maintain activity and integrity. General best-practice guidelines applicable to Recombinant Human C4orf17 Protein include:

• Storage: Store lyophilized or liquid protein preparations at the temperature specified on the product datasheet (typically –20 °C or –80 °C). Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Reconstitution: If supplied in lyophilized form, reconstitute in the buffer recommended in the datasheet. Allow the vial to equilibrate to room temperature before opening. Centrifuge briefly to collect all material before adding reconstitution buffer.
• Aliquoting: Upon reconstitution, prepare single-use aliquots to minimize freeze–thaw cycles.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) may be recommended to stabilize dilute protein solutions; confirm with the datasheet.

Always consult the product-specific datasheet for definitive storage and reconstitution instructions, as conditions are optimized per product.