Biological Background of C8G

Complement component 8 (C8) is a key protein in the terminal complement pathway and forms part of the membrane attack complex (MAC). C8 is a heterotrimer composed of three subunits: alpha (C8A), beta (C8B), and gamma (C8G). The gamma subunit, encoded by the C8G gene, is a lipocalin-family protein that associates non-covalently with the alpha subunit. While the alpha and beta chains are responsible for membrane insertion, the gamma subunit is thought to modulate the assembly and function of C8 within the MAC. Deficiency in any C8 subunit can result in impaired MAC formation and increased susceptibility to Neisseria infections in affected individuals.

Product Description and Specifications

Recombinant Human C8G Protein (SKU: rp369340) is supplied by Aladdin Scientific with reference to absea catalog number PP-25329. This recombinant protein represents the gamma subunit of human complement component C8 and is intended for use in research applications. Researchers should consult the product datasheet for confirmed details on expression host, tag configuration, molecular weight, purity grade, and formulation, as these specifications are subject to lot-to-lot variation and are best verified against the current certificate of analysis (CoA).

For the most up-to-date specifications, including SDS-PAGE purity data and endotoxin levels, please refer to the official Aladdin Scientific product page or contact technical support.

Storage, Handling, and Stability

Recombinant proteins generally require careful handling to maintain activity and integrity. As a general guideline for recombinant proteins of this class:

• Upon receipt, briefly centrifuge the vial before opening to collect any residual material from the cap or sides.
• Reconstitute lyophilized material (if applicable) using the buffer specified in the product datasheet, typically sterile phosphate-buffered saline (PBS) or a carrier-protein-containing buffer.
• For short-term storage (up to 1 month), aliquots may be kept at 4 °C. For long-term storage, aliquots should be stored at −20 °C or −80 °C to preserve activity.
• Avoid repeated freeze-thaw cycles, as these can degrade protein integrity and reduce biological activity.

Note: Always refer to the specific product datasheet for confirmed storage conditions for this lot.

Research Applications

Recombinant Human C8G Protein is suited for a range of in vitro research applications related to the complement system. Typical uses include:

• Complement pathway studies: Investigating the role of the C8 gamma subunit in MAC assembly and regulation.
• Binding and interaction assays: Studying protein-protein interactions between C8G and other complement components such as C8A.
• Immunoassay development: Use as a standard or positive control in ELISAs or Western blot assays targeting C8G.
• Structural biology: Supporting crystallography or biophysical studies of the lipocalin fold of C8G.
• C8 deficiency research: Functional reconstitution experiments to understand the consequences of C8G deficiency.

This product is intended for research use only (RUO) and is not approved for diagnostic or therapeutic applications.

Ordering and Technical Support

Recombinant Human C8G Protein is available from Aladdin Scientific under SKU rp369340. To place an order or request a quotation, contact your local Aladdin Scientific representative or use the online ordering portal on the Aladdin Scientific website.

For technical questions regarding reconstitution, application optimization, or compatibility with specific assay formats, please reach out to the Aladdin Scientific technical support team. Custom formulations or bulk quantities may be available upon request.

Researchers requiring a Safety Data Sheet (SDS), Certificate of Analysis (CoA), or product datasheet can download these documents directly from the product page or request them from customer service.