What Is Recombinant Human CACNB2 Protein?

CACNB2 (Calcium Channel, Voltage-Dependent, Beta-2 Subunit) is an auxiliary regulatory subunit of the high-voltage-activated calcium channel complex. In native physiology, the beta subunits of voltage-gated calcium channels (VGCCs) associate with the pore-forming alpha-1 subunit and modulate channel trafficking to the plasma membrane, gating kinetics, and overall channel activity.

CACNB2 is expressed prominently in cardiac muscle and neurons, where it plays a critical role in excitation-contraction coupling, neurotransmitter release, and neuronal excitability. Mutations in CACNB2 have been associated with Brugada syndrome, short QT syndrome, and certain psychiatric conditions, making this protein of significant biomedical interest.

Recombinant Human CACNB2 Protein (SKU: rp370159) from Aladdin Scientific is produced using recombinant expression systems to deliver a research-grade protein suitable for in vitro biochemical and cell-based studies.

Biological Function and Research Significance of CACNB2

The CACNB2 protein performs several important molecular functions within the calcium channel complex:

• Channel trafficking: CACNB2 facilitates the export of the alpha-1 subunit from the endoplasmic reticulum and its anchoring at the plasma membrane, increasing the density of functional channels at the cell surface.
• Gating modulation: It shifts the voltage dependence of activation and inactivation, regulates the rate of inactivation, and enhances peak calcium current amplitude.
• Protein–protein interactions: CACNB2 interacts with the alpha-1 subunit via a conserved alpha-interaction domain (AID–ABP interaction), a well-characterized protein interface relevant to drug discovery.

Because of its role in cardiac electrophysiology and neuronal signaling, CACNB2 is a relevant target in research related to arrhythmias, heart failure, epilepsy, chronic pain, and psychiatric disorders. Recombinant CACNB2 protein enables biochemical assays, binding studies, structural investigations, and antibody validation.

Recommended Applications and Usage Guidelines

Recombinant Human CACNB2 Protein (rp370159) is intended for in vitro research use only and is not approved for diagnostic or therapeutic applications. Typical research applications include:

• Protein–protein interaction studies: Investigating the binding between CACNB2 and the alpha-1 subunit AID peptide, or identifying novel interaction partners using pull-down or co-immunoprecipitation assays.
• Biochemical assays: Enzyme-linked immunosorbent assay (ELISA) development, western blot standards, and dot-blot assays.
• Antibody development and validation: Serving as an antigen for immunization or as a positive control in immunoassays.
• Structural and biophysical studies: Suitable for use in assays such as surface plasmon resonance (SPR) or isothermal titration calorimetry (ITC) to characterize binding interactions.
• Drug screening: Evaluating small-molecule modulators that target the CACNB2–alpha-1 subunit interface.

Always reconstitute and handle recombinant proteins according to the product datasheet provided with the specific lot. Avoid repeated freeze-thaw cycles to maintain protein integrity and activity.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are critical to maintaining their functional integrity. The following general best practices apply to Recombinant Human CACNB2 Protein (rp370159); always defer to the specific lot Certificate of Analysis (CoA) and product datasheet for authoritative storage conditions:

• Short-term storage: Store at 4 °C if the protein will be used within a few days of receipt.
• Long-term storage: Aliquot and store at –20 °C or –80 °C for extended stability. The addition of a carrier protein (e.g., 0.1% BSA) to the working aliquot is recommended to prevent adsorption to tube surfaces and to maintain activity during freeze-thaw.
• Avoid repeated freeze-thaw cycles: Each freeze-thaw cycle can lead to protein aggregation and loss of activity. Prepare single-use aliquots before freezing.
• Reconstitution: If supplied in lyophilized form, reconstitute in the recommended buffer as specified in the datasheet. Allow the vial to equilibrate to room temperature before opening.

Do not use protein preparations that appear turbid or have been stored improperly. Contact Aladdin Scientific customer service if you have concerns about product integrity upon receipt.

Quality, Purity, and Regulatory Information

Aladdin Scientific's Recombinant Human CACNB2 Protein (rp370159) is manufactured as a research-grade reagent. Key quality considerations include:

• Purity: Recombinant proteins in this product line are typically analyzed by SDS-PAGE and/or HPLC to confirm purity. Refer to the specific lot CoA for the exact purity value determined for your product.
• Identity confirmation: Protein identity is typically confirmed by mass spectrometry or western blot using a validated anti-CACNB2 antibody.
• Endotoxin testing: Endotoxin levels are evaluated using the Limulus Amebocyte Lysate (LAL) assay. Consult the CoA for lot-specific endotoxin data.
• Research use only: This product is strictly for laboratory research and is not intended for use in humans, clinical diagnostics, or therapeutic applications.

For procurement teams: bulk quantities and custom formulations may be available upon request. Contact Aladdin Scientific sales for pricing and lead times.