What Is CDC42BPB and Why Is It Scientifically Important?

CDC42BPB (CDC42-binding protein kinase beta), also known as MRCKbeta (myotonic dystrophy kinase-related CDC42-binding kinase beta), is a serine/threonine protein kinase that belongs to the DMPK (myotonic dystrophy protein kinase) family. It functions as a downstream effector of the small Rho GTPase CDC42 and plays a central role in regulating the actin cytoskeleton and actomyosin contractility.

By phosphorylating myosin light chain (MLC) and inhibiting myosin light-chain phosphatase (through MYPT1 phosphorylation), CDC42BPB promotes F-actin bundling, lamellipodia formation, and directed cell migration. These activities make CDC42BPB relevant to research areas including cell polarity, cancer cell invasion, neuronal morphogenesis, and cytoskeletal mechanobiology.

Recombinant Human CDC42BPB Protein from Aladdin Scientific (rp371440) provides researchers with a defined tool to investigate these kinase-dependent mechanisms in cell-free and reconstituted biochemical assays.

Expression System, Purity, and Quality Specifications

Recombinant proteins can be produced in several expression systems—each with distinct advantages for downstream applications. CDC42BPB is a large, multi-domain kinase, and the choice of expression host significantly affects protein folding, post-translational modifications, and enzymatic activity.

Expression host: Specific expression system details for rp371440 are not listed in the current source material. Researchers requiring this information—such as whether the protein is produced in E. coli, baculovirus/insect cells, or mammalian cells—should consult the product datasheet available on the Aladdin Scientific website or contact technical support directly.

Tag and purification: Recombinant CDC42BPB proteins are commonly produced with affinity tags (e.g., His-tag or GST-tag) to facilitate purification by affinity chromatography. Specific tag and purity information for rp371440 should be verified in the product datasheet.

Quality control: Aladdin Scientific applies standard QC measures to recombinant protein products, which may include SDS-PAGE for purity assessment, Western blot for identity confirmation, and activity assays where applicable. Please refer to the Certificate of Analysis (CoA) supplied with each lot for lot-specific data.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are essential to preserve the activity and integrity of recombinant proteins. The following general best practices apply to Recombinant Human CDC42BPB Protein (rp371440); always cross-reference with the product-specific datasheet for confirmed conditions.

• Long-term storage: Recombinant proteins are typically stored at −70 °C or −80 °C for long-term stability. Storage at −20 °C may be appropriate for shorter periods, depending on formulation.
• Avoid freeze-thaw cycles: Repeated freezing and thawing can denature the protein and reduce enzymatic activity. Upon first thaw, aliquot the protein into single-use volumes before re-freezing.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended on the datasheet (commonly sterile water or a defined protein storage buffer containing glycerol or BSA as stabilizers). Allow the vial to equilibrate to room temperature before opening, and dissolve gently without vortexing.
• Working concentration: Determine empirically for each assay. Pilot experiments at a range of protein concentrations are advised to establish linearity and optimal signal.
• Carrier protein: Adding BSA (0.1–0.5%) to dilution buffers can help prevent adsorptive losses at low protein concentrations.

Applications in Research: How to Use CDC42BPB Recombinant Protein

Recombinant Human CDC42BPB Protein is suited to a variety of in vitro biochemical and cell-free research applications. Typical uses include:

• Kinase activity assays: CDC42BPB phosphorylates substrates including myosin regulatory light chain (MLC2) and MYPT1. Radioactive (³²P-ATP) or non-radioactive (ADP-Glo™, TR-FRET) kinase assay formats can be used to measure its catalytic activity and screen for inhibitors.
• Inhibitor screening and drug discovery: As a kinase implicated in tumor cell invasiveness, CDC42BPB is a target of interest in oncology research. Recombinant CDC42BPB protein supports biochemical inhibitor profiling and IC₅₀ determination.
• Protein–protein interaction studies: CDC42BPB interacts with activated CDC42 via its CRIB (Cdc42/Rac interactive binding) domain. Pull-down, co-immunoprecipitation, or surface plasmon resonance (SPR) assays can characterize these interactions using the recombinant protein.
• Structural and biophysical studies: Purified recombinant protein can support crystallography, cryo-EM sample preparation, or hydrogen-deuterium exchange mass spectrometry (HDX-MS) studies.
• Antibody validation: Recombinant CDC42BPB serves as a positive control antigen for validating anti-CDC42BPB antibodies in Western blot, ELISA, and dot-blot formats.

All applications listed are for research use only and have not been validated for clinical or diagnostic purposes.

Ordering, Availability, and Technical Support

How to order: Recombinant Human CDC42BPB Protein is available from Aladdin Scientific under SKU rp371440. Orders can be placed through the Aladdin Scientific online store or by contacting your regional sales representative. Bulk quantities and custom formulations may be available upon request.

Datasheet and documentation: The full product datasheet—including confirmed expression host, tag, purity, formulation, storage conditions, and quality control data—is available on the product page. A Certificate of Analysis (CoA) is provided with each shipment and can also be requested by lot number through customer service.

Technical support: For application-specific questions, protocol guidance, or troubleshooting assistance, contact the Aladdin Scientific technical support team. Please have your SKU (rp371440) and lot number ready when reaching out to expedite support.

Shipping and storage upon receipt: Recombinant proteins are typically shipped on dry ice. Upon receipt, immediately transfer to the appropriate storage temperature as specified on the datasheet. Inspect the product for any signs of damage or thawing before use.