What Is CDHR1 and Why Is It Scientifically Important?

CDHR1 (Cadherin-Related Family Member 1), also known as photoreceptor cadherin (PCDH21), is a transmembrane glycoprotein belonging to the cadherin superfamily. It is predominantly expressed in the photoreceptor cells of the retina, particularly in the connecting cilium and outer segment disc membranes of rod and cone photoreceptors.

CDHR1 plays a critical role in photoreceptor outer segment morphogenesis and disc shedding. Mutations in the CDHR1 gene have been associated with autosomal recessive cone-rod dystrophy and retinitis pigmentosa, making it a target of significant interest in the inherited retinal dystrophy research community.

Recombinant Human CDHR1 Protein enables researchers to study CDHR1 structure, binding interactions, and function in a controlled in vitro setting, supporting research into disease mechanisms and potential therapeutic strategies.

What Are the Typical Applications of Recombinant Human CDHR1 Protein?

Recombinant Human CDHR1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: CDHR1 is known to interact with partners at the photoreceptor outer segment; recombinant protein facilitates pull-down and co-immunoprecipitation assays.
• ELISA and immunoassay development: Use as a standard or coating antigen to develop or validate detection assays.
• Antibody production and validation: Serves as an immunogen or as a positive control antigen for anti-CDHR1 antibody characterization.
• Structural and biophysical studies: Supports circular dichroism, SPR (surface plasmon resonance), and other binding or folding studies.
• Cell-based functional assays: Can be applied exogenously to study receptor-ligand interactions in retinal cell models.

Researchers should confirm suitability for their specific assay format by consulting the product datasheet or contacting Aladdin Scientific technical support.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are essential to maintain the biological activity and integrity of Recombinant Human CDHR1 Protein. General best-practice guidelines for recombinant proteins of this class include:

• Storage temperature: Store lyophilized protein at –20 °C or –80 °C for long-term stability. Avoid repeated freeze–thaw cycles.
• Reconstitution: Reconstitute lyophilized protein in sterile deionized water or an appropriate buffer (e.g., PBS) to achieve the recommended working concentration. Allow the vial to equilibrate to room temperature before opening.
• Working aliquots: Prepare single-use aliquots after reconstitution to minimize degradation caused by freeze–thaw cycling.
• Carrier protein: If the formulation does not contain a stabilizer, consider adding BSA (0.1% final concentration) to prevent adsorption losses during storage.
• Expiry and QC: Check the Certificate of Analysis (CoA) provided with each lot for specific storage conditions, purity data, and expiry date.

Note: Always refer to the specific product datasheet for rp365094 for lot-specific instructions, as formulation details may vary.

Quality, Purity, and Regulatory Information

Aladdin Scientific's Recombinant Human CDHR1 Protein (rp365094) is manufactured and quality-controlled to research-grade standards. Key quality attributes typically assessed for recombinant proteins include:

• Purity: Assessed by SDS-PAGE and/or HPLC. Research-grade recombinant proteins are typically ≥85–95% pure; refer to the lot-specific CoA for the confirmed value.
• Biological activity: Functional activity may be confirmed via binding assays or other activity tests; see the product datasheet for activity data where available.
• Endotoxin level: Endotoxin content is tested (commonly by LAL assay) to ensure suitability for sensitive cell-based assays.
• Identity confirmation: Protein identity is typically verified by Western blot or mass spectrometry.

This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or clinical use. Compliance with institutional biosafety guidelines is the responsibility of the end user.

Ordering and Technical Support

Recombinant Human CDHR1 Protein is available from Aladdin Scientific under SKU rp365094. To place an order or inquire about availability, pricing, or bulk quantities, please visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, buffer compatibility, or assay optimization, Aladdin Scientific's technical support team is available to assist. Please have your lot number and CoA ready when contacting support for the most efficient service.

Custom formulations or larger quantities may be available upon request — contact Aladdin Scientific directly to discuss your specific requirements.