What Is CENPH and Why Is It Important in Research?

CENPH (Centromere Protein H) is a constitutive centromere-associated protein that forms part of the CENPA-NAC (CENPA Nucleosome-Associated Complex). This complex is essential for the recruitment of outer kinetochore proteins and is indispensable for faithful chromosome segregation during mitosis and meiosis. Disruption of CENPH function has been associated with mitotic errors and chromosomal instability, making it a protein of interest in cancer biology and cell division research. Recombinant Human CENPH Protein produced by Aladdin Scientific (SKU: rp365908) provides researchers with a defined, high-quality reagent to investigate these mechanisms in vitro.

Handling, Reconstitution, and Storage Guidelines

Proper handling of Recombinant Human CENPH Protein is critical to maintaining its activity and integrity. Upon receipt, centrifuge the vial briefly before opening to consolidate the contents. Reconstitute lyophilized protein in sterile, ultrapure water or an appropriate buffer as specified in the product datasheet. It is strongly recommended to prepare single-use aliquots immediately after reconstitution to minimize the number of freeze-thaw cycles, as repeated freezing and thawing can significantly reduce protein activity. Store reconstituted protein at -80°C for long-term preservation and at -20°C for short-term use. Avoid storing the protein in frost-free freezers that cycle temperature automatically.

Note: Always consult the product-specific datasheet (reference: absea#PP-387) for confirmed buffer composition, concentration, and reconstitution volume, as these parameters are lot-specific and take precedence over general guidance.

Research Applications of Recombinant Human CENPH Protein

Recombinant Human CENPH Protein is suitable for a variety of in vitro research applications, including:

• Protein–protein interaction studies: CENPH is a key node in the CENPA-NAC complex; recombinant protein enables pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) binding experiments.
• Antibody specificity validation: Use as a positive control antigen to confirm antibody selectivity for CENPH in western blotting or ELISA workflows.
• Functional/biochemical assays: Study the role of CENPH in centromere maintenance and kinetochore formation using cell-free or reconstituted systems.
• Structural biology: Suitable as a component for complex reconstitution experiments aimed at elucidating the architecture of the inner kinetochore.

Researchers in cancer biology, chromosome biology, and mitosis research will find this reagent particularly useful given CENPH's known roles in chromosomal stability.

Quality, Purity, and Verification

High purity is essential when using recombinant proteins in sensitive biochemical assays. For Recombinant Human CENPH Protein (rp365908), researchers should refer to the Certificate of Analysis (CoA) and the product datasheet (absea#PP-387) for lot-specific purity data, SDS-PAGE validation images, and endotoxin levels. When evaluating any recombinant protein, key quality indicators include:

• Purity: Typically assessed by SDS-PAGE and/or HPLC; a high-quality research-grade protein should ideally be >90% pure.
• Endotoxin content: Critical for cell-based assays; refer to CoA for actual measured values.
• Biological activity: Where applicable, confirmed by functional assay data provided on the datasheet.

Contact Aladdin Scientific's technical support team for lot-specific CoA documents or additional technical data.