What Is CORO1A? Biological Background

CORO1A (Coronin-1A) is a member of the evolutionarily conserved coronin family of WD-repeat–containing, actin-associated proteins. In humans, CORO1A is encoded by the CORO1A gene and is most highly expressed in hematopoietic cells, particularly T lymphocytes, neutrophils, and macrophages.

Structurally, Coronin-1A contains an N-terminal WD-repeat beta-propeller domain responsible for actin filament binding, a unique region, and a C-terminal coiled-coil domain that mediates oligomerization. These domains collectively enable CORO1A to regulate actin polymerization and branching by modulating the Arp2/3 complex, as well as by interacting with filamentous actin (F-actin) directly.

Beyond cytoskeletal remodeling, CORO1A plays pivotal roles in phagolysosome maturation, cell migration, and the survival signaling of naive T cells. Loss-of-function mutations in CORO1A are associated with a form of primary immunodeficiency characterized by T cell lymphopenia in humans, underscoring its non-redundant biological importance.

Recombinant Human CORO1A Protein: Key Applications

Recombinant Human CORO1A Protein (rp365890) is a valuable research tool across multiple experimental contexts:

• Protein–protein interaction studies: CORO1A's interaction with Arp2/3, F-actin, and various signaling adapters can be interrogated using pull-down, co-immunoprecipitation, or surface plasmon resonance assays with the recombinant protein as bait or prey.
• Biochemical and enzymatic assays: The recombinant protein can be used in actin polymerization or depolymerization assays to characterize how CORO1A modulates cytoskeletal dynamics in vitro.
• Antibody validation: Recombinant CORO1A serves as a positive control antigen for Western blot, ELISA, and immunoprecipitation to validate anti-CORO1A antibodies.
• Structural studies: High-purity recombinant protein is suitable as a starting material for crystallography or cryo-EM studies aimed at resolving CORO1A domain architecture.
• Immune cell research: Researchers studying T cell homeostasis, thymic egress, or macrophage–pathogen interactions can use recombinant CORO1A to dissect molecular mechanisms in reconstitution or cell-free systems.

Protein Specifications & Quality Considerations

When selecting a recombinant protein for critical assays, researchers should evaluate the following parameters for Recombinant Human CORO1A Protein (rp365890):

• Expression system: The choice of host (e.g., E. coli, baculovirus/insect cells, or mammalian HEK293 cells) affects post-translational modifications and protein folding. Researchers requiring native-like glycosylation or phosphorylation should confirm the expression platform with Aladdin Scientific.
• Purity: For structural or quantitative binding assays, purity ≥90% (assessed by SDS-PAGE) is generally recommended. Confirm purity certification data in the product datasheet.
• Tag information: Recombinant proteins may carry affinity tags (His, GST, Flag, etc.) that can influence assay design, particularly in pull-down experiments. Verify tag position and cleavability as needed.
• Activity validation: Where applicable, functional activity data (e.g., actin-binding or Arp2/3 inhibition assays) in the product datasheet supports confidence in protein integrity.
• Storage and stability: Recombinant proteins should be stored according to datasheet recommendations, typically at −20°C or −80°C with carrier protein (e.g., BSA) and avoidance of repeated freeze–thaw cycles.

For full technical specifications, refer to the official product datasheet available on the Aladdin Scientific website.

CORO1A in Disease & Translational Research

CORO1A has attracted growing interest as a disease-relevant protein in several research areas:

Primary Immunodeficiency: Biallelic loss-of-function mutations in CORO1A cause a rare combined immunodeficiency characterized by severe T cell lymphopenia, recurrent infections, and in some cases, Epstein–Barr virus–associated lymphoproliferation. Recombinant CORO1A supports mechanistic studies of these conditions.

Mycobacterial Infection: CORO1A (originally identified as TACO — Tryptophan Aspartate-containing Coat protein) was described in the context of Mycobacterium tuberculosis survival within macrophages. The pathogen exploits CORO1A to prevent phagolysosome fusion, making CORO1A a potential target in anti-tuberculosis research.

Cancer Biology: Dysregulation of CORO1A expression has been reported in certain cancers. Its role in cell migration and cytoskeletal remodeling positions it as a candidate modulator of tumor invasion and metastasis.

Autoimmunity: Given CORO1A's role in T cell signaling and homeostasis, it is studied in the context of autoimmune conditions where aberrant T cell activity is central to pathogenesis.

Recombinant Human CORO1A Protein (rp365890) provides researchers with a consistent, well-defined reagent to advance studies in all of these therapeutic areas.

Ordering & Storage Information

Recombinant Human CORO1A Protein is available from Aladdin Scientific under SKU rp365890. Research teams can place orders directly through the Aladdin Scientific online store or by contacting the sales team for bulk or custom requirements.

Recommended storage conditions for recombinant proteins generally include:

• Short-term (≤1 week): 4°C
• Long-term: −20°C or −80°C, aliquoted to minimize freeze–thaw cycles
• Addition of a carrier protein such as 0.1% BSA is advisable for dilute solutions

Please refer to the product-specific datasheet provided with shipment or available on the Aladdin Scientific product page for definitive storage, formulation, and handling instructions. For technical support, contact Aladdin Scientific's scientific support team.