What Is Recombinant Human FANCM Protein?

Recombinant Human FANCM Protein (SKU: rp366912) is a recombinant form of the human FANCM gene product, a member of the Fanconi anemia (FA) complementation group. FANCM encodes an ATP-dependent DNA helicase and branch-point translocase that acts as a central component of the FA core complex. This complex is essential for the monoubiquitination of FANCD2 and FANCI, which in turn activates downstream DNA repair processes to resolve DNA interstrand crosslinks (ICLs).

FANCM is unique among FA proteins because it directly engages DNA substrates via its DEAH-box helicase domain and can remodel stalled replication forks. Its scaffold function recruits additional FA and non-FA repair factors to sites of DNA damage, making it indispensable for maintaining genomic integrity.

What Are the Key Research Applications?

Recombinant Human FANCM Protein is suitable for a range of biochemical and cell biology research applications, including:

• In vitro helicase and ATPase activity assays: Characterizing the enzymatic properties of FANCM or testing the effect of small-molecule inhibitors on its activity.
• Protein–protein interaction studies: Investigating FANCM interactions with FA core complex partners (e.g., FAAP24, MHF1/MHF2) using pull-down or co-immunoprecipitation assays.
• DNA-binding and branch migration assays: Using synthetic Holliday junction or replication fork substrates to study FANCM translocase activity.
• Western blotting and ELISA standards: As a positive control or standard for immunodetection assays.

Note: Verify specific validated applications on the product datasheet prior to use.

Storage, Handling, and Quality Considerations

Proper storage and handling are critical to preserving the activity and integrity of Recombinant Human FANCM Protein:

• Temperature: Store at -80 °C upon receipt. Short-term storage at -20 °C may be acceptable depending on formulation; consult the product datasheet for confirmed conditions.
• Freeze-thaw cycles: Minimize freeze-thaw cycles by preparing single-use aliquots. Repeated freezing and thawing can denature the protein and reduce enzymatic activity.
• Carrier protein: If the formulation includes BSA or glycerol as a stabilizer, ensure compatibility with your downstream assay before use.
• Purity and quality control: Recombinant proteins from Aladdin Scientific undergo SDS-PAGE and/or other QC analyses to confirm expected molecular weight and purity. Refer to the Certificate of Analysis (CoA) for lot-specific data.

Flag: Specific storage buffer composition, purity percentage, and molecular weight should be verified against the official product datasheet and CoA for SKU rp366912.

Ordering and Technical Support

Recombinant Human FANCM Protein (SKU: rp366912) is available for order directly from Aladdin Scientific. To place an order or request a quote:

• Visit the Aladdin Scientific website and search by SKU rp366912.
• Contact your regional Aladdin Scientific sales representative for bulk pricing, custom formulations, or special requirements.
• For technical questions regarding experimental design, protein activity, or compatibility, reach out to Aladdin Scientific's technical support team.

Certificates of Analysis (CoA) and Safety Data Sheets (SDS) are available on the product page or upon request from customer service. For returns or product quality concerns, please refer to Aladdin Scientific's return and refund policy.